The neuropeptide VIP regulates the expression of the tyrosine hydroxylase gene in cultured avian sympathetic neurons
Details
Serval ID
serval:BIB_E5AA045B7A99
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The neuropeptide VIP regulates the expression of the tyrosine hydroxylase gene in cultured avian sympathetic neurons
Journal
Brain Research. Molecular Brain Research
ISSN
0169-328X (Print)
Publication state
Published
Issued date
10/1993
Volume
20
Number
1-2
Pages
125-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Research Support, Non-U.S. Gov't --- Old month value: Oct
Abstract
The neuropeptide vasoactive intestinal polypeptide (VIP) increases the activity of tyrosine hydroxylase (TH), the rate-limiting enzyme in the biosynthesis of catecholamines, in cultured chicken sympathetic neurons. We report here that VIP acts by increasing TH mRNA levels in these cells. Induction of TH mRNA is transient and reaches maximal values 6-8 h after the addition of the peptide to the cultures. TH mRNA levels return to control values after 1-2 days. The quail cDNA probe detects a single mRNA species of approximately 9 kb in RNA extracted both from embryonic chicken sympathetic neurons and adult quail adrenal medulla.
Keywords
Animals
Cells, Cultured
Chick Embryo
Gene Expression Regulation/*physiology
RNA, Messenger/metabolism
Sympathetic Nervous System/cytology/*physiology
Tyrosine 3-Monooxygenase/*genetics
Vasoactive Intestinal Peptide/*physiology
Pubmed
Create date
28/01/2008 9:44
Last modification date
20/08/2019 17:09
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