Pyruvate modifies glycolytic and oxidative metabolism of rat embryonic spinal cord astrocyte cell lines and prevents their spontaneous transformation.

Details

Serval ID
serval:BIB_E5A77A074349
Type
Article: article from journal or magazin.
Collection
Publications
Title
Pyruvate modifies glycolytic and oxidative metabolism of rat embryonic spinal cord astrocyte cell lines and prevents their spontaneous transformation.
Journal
Journal of Neurochemistry
Author(s)
Rouleau C., Rakotoarivelo C., Petite D., Lambert K., Fabre C., Bonardet A., Mercier J., Baldet P., Privat A., Langley K., Mersel M.
ISSN
0022-3042 (Print)
ISSN-L
0022-3042
Publication state
Published
Issued date
2007
Volume
100
Number
6
Pages
1589-1598
Language
english
Abstract
This study aimed to provide detailed data on mitochondrial respiration of normal astrocyte cell lines derived from rat embryonic spinal cord. Astrocytes in early passages (EP), cultured without pyruvate for more than 35 passages, defined here as late passages (LP), undergo spontaneous transformation. To study initial steps in cell transformation, EP data were compared with those of LP cells. LP cells had reduced glycolysis, fewer mitochondria and extremely low oxidative rates, resulting from a dysfunction of complexes I and II + III of the respiratory chain. Treatment of EP cells with pyruvate until they were, by definition, LP cultures prevented transformation of these cells. Pyruvate-treated EP cells had more mitochondria than normal cells but slightly lower respiratory rates. The increase of mitochondrial content thus appears to act as a compensatory effect to maintain oxidative phosphorylation in these LP 'non-transformed' cells, in which mitochondrial function is reduced. However, pyruvate treatment of transformed LP cells during additional passages did not significantly restore their oxidative metabolism. These data highlight changes accompanying spontaneous astrocyte transformation and suggest potential targets for the control of astrocyte proliferation and reaction to various insults to the central nervous system.
Keywords
Aging/drug effects, Aging/physiology, Animals, Astrocytes/drug effects, Astrocytes/ultrastructure, Cells, Cultured, Electron Transport Chain Complex Proteins/metabolism, Embryo, Mammalian, Glycolysis/drug effects, Mitochondria/drug effects, Oxygen Consumption/drug effects, Pyruvic Acid/metabolism, Pyruvic Acid/pharmacology, Rats, Spinal Cord/cytology
Pubmed
Create date
30/09/2011 13:53
Last modification date
20/08/2019 16:09
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