Anti-HLA antibody repertoire after IVIg infusion in highly sensitized patients waiting for kidney transplantation.

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State: Public
Version: Final published version
Serval ID
serval:BIB_E587F1120887
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Anti-HLA antibody repertoire after IVIg infusion in highly sensitized patients waiting for kidney transplantation.
Journal
Swiss Medical Weekly
Author(s)
Ferrari-Lacraz Sylvie, Aubert Vincent, Buhler Leo, Pascual Manuel, Andresen Irmgard, Binet Isabelle, Martin Pierre-Yves, Villard Jean
ISSN
1424-7860 (Print)
ISSN-L
0036-7672
Publication state
Published
Issued date
2006
Volume
136
Number
43-44
Pages
696-702
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Polyclonal intravenous immunoglobulin (IVIg) treatment reduces crossmatch positivity and increases rates of transplantation in highly sensitised patients (HS). We quantified the panel reactive antibody (PRA) by microlymphocytotoxicity (MLCC), and we analysed anti-HLA class I and class II IgG specific antibody repertoire by Luminex before and after IVIg infusion alone in HS patients awaiting kidney transplantation. Five patients received three monthly infusions of 1 g/kg of IVIg. Serum samples collected pre and post IVIg treatment were submitted for PRA analysis by MLCC. Anti-class I and anti-class II antibody specificities were then tested by Luminex. We focused on the anti-HLA class I and class II antibodies directed against HLA expressed by a previous graft. We also analysed the anti-HLA antibody repertoire in three patients who had not received IVIg infusion. The PRA level determined by MLCC decreased significantly in one of the five patients, dropping from 40% to 17%. The Luminex assay showed fluctuations of the anti-HLA antibody levels over time, but no significant longterm modifications of the anti-HLA antibody repertoire were observed, even in the patient with a strong and prolonged reduction of the PRA determined by MLCC. Our results show that IVIg at 1 g/kg is not sufficient to reduce PRA and does not modify the repertoire of specific anti-HLA antibody determined by Luminex.
Keywords
Adult, Aged, Antibodies/blood, Case-Control Studies, Female, Graft Rejection/prevention & control, HLA Antigens/immunology, Humans, Immunoglobulins, Intravenous/therapeutic use, Kidney Transplantation/immunology, Male, Middle Aged, Pilot Projects, Transplantation Conditioning/methods
Pubmed
Web of science
Create date
05/01/2011 10:23
Last modification date
20/08/2019 16:08
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