Comparative molecular modelling of the Fas-ligand and other members of the TNF family

Details

Serval ID
serval:BIB_E5831B58C8B7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Comparative molecular modelling of the Fas-ligand and other members of the TNF family
Journal
Molecular Immunology
Author(s)
Peitsch  M. C., Tschopp  J.
ISSN
0161-5890 (Print)
Publication state
Published
Issued date
07/1995
Volume
32
Number
10
Pages
761-72
Notes
Comparative Study
Journal Article --- Old month value: Jul
Abstract
A number of proteins with significant similarity to the tumour necrosis factor (TNF) have been identified over the last years. Upon interaction with their cognate receptor (members of the TNF-receptor family), all members of this protein family induce either cell death or proliferation/differentiation of the receptor-bearing cells. One of the last identified members of the TNF family is the apoptosis-inducing ligand of the Fas-receptor, termed Fas-ligand (FasL). Here we report the cloning and sequencing of the mouse cDNA for the FasL. Using knowledge-based protein modelling, we demonstrate that all members of the TNF family form trimeric complexes, and define the residues located at the subunit interfaces. The resulting structurally corrected multiple sequence alignment allows the identification of residues potentially involved in receptor recognition, and should help design mutagenesis experiments for structure-function relationship studies.
Keywords
Amino Acid Sequence Animals Antigens, CD95 Antigens, Surface/metabolism Cloning, Molecular DNA, Complementary/genetics/isolation & purification Fas Ligand Protein Membrane Glycoproteins/*chemistry/genetics/metabolism Mice Models, Molecular Molecular Sequence Data Protein Structure, Secondary Sequence Alignment Tumor Necrosis Factor-alpha/*chemistry/metabolism
Pubmed
Web of science
Create date
24/01/2008 15:18
Last modification date
20/08/2019 16:08
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