In vivo reprogramming of circuit connectivity in postmitotic neocortical neurons.

Details

Serval ID
serval:BIB_E546405F8816
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
In vivo reprogramming of circuit connectivity in postmitotic neocortical neurons.
Journal
Nature Neuroscience
Author(s)
De la Rossa A., Bellone C., Golding B., Vitali I., Moss J., Toni N., Lüscher C., Jabaudon D.
ISSN
1546-1726 (Electronic)
ISSN-L
1097-6256
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
16
Number
2
Pages
193-200
Language
english
Abstract
The molecular mechanisms that control how progenitors generate distinct subtypes of neurons, and how undifferentiated neurons acquire their specific identity during corticogenesis, are increasingly understood. However, whether postmitotic neurons can change their identity at late stages of differentiation remains unknown. To study this question, we developed an electrochemical in vivo gene delivery method to rapidly manipulate gene expression specifically in postmitotic neurons. Using this approach, we found that the molecular identity, morphology, physiology and functional input-output connectivity of layer 4 mouse spiny neurons could be specifically reprogrammed during the first postnatal week by ectopic expression of the layer 5B output neuron-specific transcription factor Fezf2. These findings reveal a high degree of plasticity in the identity of postmitotic neocortical neurons and provide a proof of principle for postnatal re-engineering of specific neural microcircuits in vivo.
Keywords
Age Factors, Animals, Animals, Newborn, Cell Cycle/drug effects, Cell Cycle/genetics, Cholera Toxin/metabolism, Cyclohexanones/pharmacology, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Dendrites/metabolism, Embryo, Mammalian, Epithelial Sodium Channels/genetics, Gene Expression Regulation, Developmental/drug effects, Gene Expression Regulation, Developmental/genetics, Green Fluorescent Proteins/genetics, Membrane Potentials/drug effects, Membrane Potentials/genetics, Mice, Mice, Transgenic, Microscopy, Electron, Transmission, Neocortex/cytology, Neocortex/embryology, Nerve Net/physiology, Nerve Tissue Proteins/genetics, Nerve Tissue Proteins/metabolism, Neural Pathways/physiology, Neurons/physiology, Neurons/ultrastructure, Patch-Clamp Techniques, Rhodopsin/genetics, Statistics, Nonparametric, Vesicular Glutamate Transport Protein 2/metabolism
Pubmed
Web of science
Create date
13/02/2013 11:58
Last modification date
20/08/2019 16:08
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