The transcription and export complex THO/TREX contributes to transcription termination in plants.

Details

Serval ID
serval:BIB_E53588AE2E12
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The transcription and export complex THO/TREX contributes to transcription termination in plants.
Journal
PLoS genetics
Author(s)
Khan G.A., Deforges J., Reis R.S., Hsieh Y.F., Montpetit J., Antosz W., Santuari L., Hardtke C.S., Grasser K.D., Poirier Y.
ISSN
1553-7404 (Electronic)
ISSN-L
1553-7390
Publication state
Published
Issued date
04/2020
Peer-reviewed
Oui
Volume
16
Number
4
Pages
e1008732
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Transcription termination has important regulatory functions, impacting mRNA stability, localization and translation potential. Failure to appropriately terminate transcription can also lead to read-through transcription and the synthesis of antisense RNAs which can have profound impact on gene expression. The Transcription-Export (THO/TREX) protein complex plays an important role in coupling transcription with splicing and export of mRNA. However, little is known about the role of the THO/TREX complex in the control of transcription termination. In this work, we show that two proteins of the THO/TREX complex, namely TREX COMPONENT 1 (TEX1 or THO3) and HYPER RECOMBINATION1 (HPR1 or THO1) contribute to the correct transcription termination at several loci in Arabidopsis thaliana. We first demonstrate this by showing defective termination in tex1 and hpr1 mutants at the nopaline synthase (NOS) terminator present in a T-DNA inserted between exon 1 and 3 of the PHO1 locus in the pho1-7 mutant. Read-through transcription beyond the NOS terminator and splicing-out of the T-DNA resulted in the generation of a near full-length PHO1 mRNA (minus exon 2) in the tex1 pho1-7 and hpr1 pho1-7 double mutants, with enhanced production of a truncated PHO1 protein that retained phosphate export activity. Consequently, the strong reduction of shoot growth associated with the severe phosphate deficiency of the pho1-7 mutant was alleviated in the tex1 pho1-7 and hpr1 pho1-7 double mutants. Additionally, we show that RNA termination defects in tex1 and hpr1 mutants leads to 3'UTR extensions in several endogenous genes. These results demonstrate that THO/TREX complex contributes to the regulation of transcription termination.
Keywords
Amino Acid Oxidoreductases/genetics, Amino Acid Oxidoreductases/metabolism, Arabidopsis/genetics, Arabidopsis/metabolism, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Gene Expression Regulation, Plant, Transcription Termination, Genetic
Pubmed
Web of science
Open Access
Yes
Create date
25/04/2020 20:06
Last modification date
18/09/2020 5:26
Usage data