Stimulation by nicotine of enteric inhibitory nerves and release of vasoactive intestinal peptide in the taenia of the guinea-pig caecum

Details

Serval ID
serval:BIB_E53326A5C855
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Stimulation by nicotine of enteric inhibitory nerves and release of vasoactive intestinal peptide in the taenia of the guinea-pig caecum
Journal
European Journal of Pharmacology
Author(s)
Iselin  C. E., Martin  J. L., Magistretti  P. J., Ferrero  J. D.
ISSN
0014-2999
Publication state
Published
Issued date
03/1988
Peer-reviewed
Oui
Volume
148
Number
2
Pages
179-86
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar 29
Abstract
The stimulation by nicotine of intramural nerves and the role of ATP and vasoactive intestinal peptide (VIP) as inhibitory transmitters were studied in the isolated taenia of the guinea-pig caecum. Nicotine (4-32 microM) caused transient, concentration-dependent relaxations which were unaffected by atropine, prazosin or sotalol. Drugs with membrane-stabilizing activity, such as dl-propranolol (0.5 microM), d-propranolol (0.5 microM) or lidocaine (10 microM) antagonized the nicotine-induced relaxation without modifying the response to electrical field stimulation. Similar results were obtained with the cyclooxygenase inhibitor, indomethacin (2.8 microM). Nucleotide pyrophosphatase (0.5 U/ml), which hydrolyzes ATP to AMP, reversibly inhibited the response to nicotine but the response to field stimulation was not decreased. Nicotine evoked a calcium-dependent release of VIP, which was blocked by tetrodotoxin (1 microM), d-propranolol (0.5 microM) or, as previously shown, by apamin (0.2 microM). The finding that nicotine-induced relaxation was accompanied by the neuronal release of VIP is compatible with the possibility that VIP is an inhibitory transmitter but is not definitive evidence, since it could have been due to the stimulation of distinct populations of nerves by nicotine.
Keywords
Animals Apamin/pharmacology Colon/innervation/metabolism/*physiology Female Guinea Pigs Male Nicotine/*pharmacology Pyrophosphatases/physiology Stimulation, Chemical Vasoactive Intestinal Peptide/*secretion
Pubmed
Web of science
Create date
20/01/2008 18:22
Last modification date
20/08/2019 16:08
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