Phylogeographical Analysis Reveals the Historic Origin, Emergence, and Evolutionary Dynamics of Methicillin-Resistant Staphylococcus aureus ST228.

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State: Public
Version: Final published version
License: CC BY-NC-ND 4.0
Serval ID
serval:BIB_E52BBB0E2952
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Phylogeographical Analysis Reveals the Historic Origin, Emergence, and Evolutionary Dynamics of Methicillin-Resistant Staphylococcus aureus ST228.
Journal
Frontiers in microbiology
Author(s)
Abdelbary MMH, Feil E.J., Senn L., Petignat C., Prod'hom G., Schrenzel J., François P., Werner G., Layer F., Strommenger B., Pantosti A., Monaco M., Denis O., Deplano A., Grundmann H., Blanc D.S.
ISSN
1664-302X (Print)
ISSN-L
1664-302X
Publication state
Published
Issued date
2020
Peer-reviewed
Oui
Volume
11
Pages
2063
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is a common healthcare-associated pathogen that remains a major public health concern. Sequence type 228 (ST228) was first described in Germany and spread to become a successful MRSA clone in several European countries. In 2000, ST228 emerged in Lausanne and has subsequently caused several large outbreaks. Here, we describe the evolutionary history of this clone and identify the genetic changes underlying its expansion in Switzerland.
We aimed to understand the phylogeographic and demographic dynamics of MRSA ST228/ST111 by sequencing 530 representative isolates of this clone that were collected from 14 European countries between 1997 and 2012.
The phylogenetic analysis revealed distinct lineages of ST228 isolates associated with specific geographic origins. In contrast, isolates of ST111, which is a single locus variant of ST228 sharing the same spa type t041, formed a monophyletic cluster associated with multiple countries. The evidence points to a German origin of the sampled population, with the basal German lineage being characterized by spa type t001. The highly successful Swiss ST228 lineage diverged from this progenitor clone through the loss of the aminoglycoside-streptothricin resistance gene cluster and the gain of mupirocin resistance. This lineage was introduced first in Geneva and was subsequently introduced into Lausanne.
Our results reveal the radiation of distinct lineages of MRSA ST228 from a German progenitor, as the clone spread into different European countries. In Switzerland, ST228 was introduced first in Geneva and was subsequently introduced into Lausanne.
Keywords
MRSA, ST228, WGS, epidemiology, genomic epidemiology, phylogeny, phylogeography
Pubmed
Web of science
Open Access
Yes
Create date
05/10/2020 13:19
Last modification date
14/10/2020 5:23
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