Comparison of five commercial serological tests for the detection of anti-Chlamydia trachomatis antibodies.

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Serval ID
serval:BIB_E4EEF5845C8D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Comparison of five commercial serological tests for the detection of anti-Chlamydia trachomatis antibodies.
Journal
European Journal of Clinical Microbiology and Infectious Diseases
Author(s)
Baud D., Regan L., Greub G.
ISSN
1435-4373[electronic], 0934-9723[linking]
Publication state
Published
Issued date
2010
Volume
29
Number
6
Pages
669-675
Language
english
Abstract
Screening for Chlamydia trachomatis-specific antibodies is valuable in investigating recurrent miscarriage, tubal infertility and extrauterine pregnancy. We compared here the performance of immunofluorescence (IF) to four other commercial tests in detecting IgG antibodies directed against C. trachomatis: two enzyme-linked immunosorbent assays (ELISAs) using the major outer membrane protein (MOMP) as the antigen, commercialised respectively by Medac and R-Biopharm (RB), one ELISA using the chlamydial heat shock protein 60 (cHSP60) as the antigen (Medac), as well as a new automated epifluorescence immunoassay (InoDiag). A total of 405 patients with (n = 251) and without (n = 154) miscarriages were tested by all five tests. The prevalence of C. trachomatis-specific IgG antibodies as determined by the IF, cHSP60-Medac, MOMP-Medac, MOMP-RB and InoDiag was 14.3, 23.2, 14.3, 11.9 and 26.2%, respectively. InoDiag exhibited the highest sensitivity, whereas MOMP-RB showed the best specificity. Cross-reactivity was observed with C. pneumoniae using IF, MOMP-RB and InoDiag, and Parachlamydia acanthamoebae using the cHSP60 ELISA test. No cross-reactivity was observed between C. trachomatis and the other Chlamydiales (Neochlamydia hartmannellae, Waddlia chondrophila and Simkania negevensis). Given its high sensitivity, the new automated epifluorescence immunoassay from InoDiag represents an interesting alternative. The MOMP-based ELISA of R-Biopharm should be preferred for large serological studies, given the high throughput of ELISA and its excellent specificity.
Keywords
Atypical Bacterial Pneumonia, Pelvic-Inflammatory-Disease, Heat-Shock-Protein, Pregnant-Women, Infections, Assays, Igg, Serodiagnosis, Immunoassays, Surveillance
Pubmed
Web of science
Open Access
Yes
Create date
31/05/2010 14:34
Last modification date
01/10/2019 6:20
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