Mutation of the Traj18 gene segment using TALENs to generate Natural Killer T cell deficient mice.

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Serval ID
serval:BIB_E4CC5332668F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Mutation of the Traj18 gene segment using TALENs to generate Natural Killer T cell deficient mice.
Journal
Scientific Reports
Author(s)
Zhang J., Bedel R., Krovi S.H., Tuttle K.D., Zhang B., Gross J., Gapin L., Matsuda J.L.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
6
Pages
27375
Language
english
Abstract
Invariant Natural Killer T (iNKT) cells are a unique subset of T lymphocytes that have been implicated in both promoting and suppressing a multitude of immune responses. In mice, iNKT cells express T cell antigen receptors (TCRs) comprising a unique TCRα rearrangement between the Trav11 and Traj18 gene segments. When paired with certain Trbv TCRβ chains, these TCRs recognize lipid antigens presented by the major histocompatibility complex (MHC) class I-like molecule, CD1d. Until recently, the sole model of iNKT deficiency targeted the Jα18, which is absolutely required to form the TCR with the appropriate antigenic specificity. However, these mice were demonstrated to have a large reduction in TCR repertoire diversity, which could confound results arising from studies using these mice. Here, we have created a new NKT-deficient mouse strain using transcription activator-like effector nuclease (TALEN) technology to only disrupt the expression of Jα18, leaving the remaining Jα repertoire unperturbed. We confirm that these mice lack iNKT cells and do not respond to lipid antigen stimulation while the development of conventional T cells, regulatory T cells, and type Ib NKT cells is normal. This new mouse strain will serve as a new model of iNKT cell deficiency to facilitate our understanding of iNKT biology.
Pubmed
Web of science
Open Access
Yes
Create date
24/06/2016 19:21
Last modification date
20/08/2019 16:08
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