Differences in the gene expression profile of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) in primary colorectal tumors and their synchronous liver metastases.

Details

Serval ID
serval:BIB_E49FB20975C1
Type
Article: article from journal or magazin.
Collection
Publications
Title
Differences in the gene expression profile of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) in primary colorectal tumors and their synchronous liver metastases.
Journal
Anticancer research
Author(s)
Gentner B., Wein A., Croner R.S., Zeittraeger I., Wirtz R.M., Roedel F., Dimmler A., Dorlaque L., Hohenberger W., Hahn E.G., Brueckl W.M.
ISSN
0250-7005 (Print)
ISSN-L
0250-7005
Publication state
Published
Issued date
01/2009
Peer-reviewed
Oui
Volume
29
Number
1
Pages
67-74
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) have been strongly implicated in the pathogenesis of many types of human cancer. We wanted to specifically define their role in established colorectal cancer liver metastases.
The MMP/TIMP expression profiles of N=9 colorectal primary tumour liver metastasis tissue pairs were determined using oligonucleotide-based arrays. Expression levels for the most relevant MMPs were confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR). Additionally, unsupervised clustering using the MMP/TIMP profile of N=25 colorectal cancer liver metastases was performed and the response to palliative 5-fluorouracil (5-FU)-based chemotherapy was assessed using radiological response criteria.
When comparing the primary tumors to their synchronous liver metastases, a statistically significant (p < 0.05) down-regulation of MMP1, -2, -3 and -12 was found in the metastases. Unsupervised clustering using the MMP/TIMP profiles of 25 liver metastases revealed two distinct subgroups with different responses to palliative, 5-FU-based chemotherapy (response rates: 22% vs. 56%, respectively). In particular, higher MMP7, TIMP1 and TIMP2 levels were found in the unfavourable group, while higher expression of MMP2, -9, -11 and -14 was associated with a more favourable response to chemotherapy.
Colorectal cancer liver metastases show a distinctive MMP/TIMP profile with predictive implications.
Keywords
Adult, Aged, Aged, 80 and over, Colorectal Neoplasms/genetics, Colorectal Neoplasms/metabolism, Colorectal Neoplasms/pathology, Colorectal Neoplasms/therapy, Female, Fluorouracil/therapeutic use, Gene Expression Profiling, Humans, Liver Neoplasms/genetics, Liver Neoplasms/metabolism, Liver Neoplasms/secondary, Liver Neoplasms/therapy, Male, Matrix Metalloproteinases/biosynthesis, Matrix Metalloproteinases/genetics, Middle Aged, Predictive Value of Tests, Reverse Transcriptase Polymerase Chain Reaction, Tissue Inhibitor of Metalloproteinases/biosynthesis, Tissue Inhibitor of Metalloproteinases/genetics
Pubmed
Web of science
Create date
13/07/2022 16:13
Last modification date
14/07/2022 5:37
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