Peptide-primed CD4+ cells and malaria sporozoites

Details

Serval ID
serval:BIB_E49A991A834F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Peptide-primed CD4+ cells and malaria sporozoites
Journal
Immunology Letters
Author(s)
Del Giudice  G., Grillot  D., Renia  L., Muller  I., Corradin  G., Louis  J. A., Mazier  D., Lambert  P. H.
ISSN
0165-2478 (Print)
Publication state
Published
Issued date
08/1990
Volume
25
Number
1-3
Pages
59-63
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug
Abstract
We have mapped a T cell epitope in the circumsporozoite (CS) protein of the murine malaria parasite, Plasmodium yoelii. A 21-mer synthetic peptide corresponding to the amino acid positions 59-79 (referred to as Py1), induced specific proliferation in BALB/c and C57BL/6 mice, and provided help for the production of antibodies to peptides from the repetitive region, (QGPGAP)n, of the same CS protein, when mice were immunized with the Py1 peptide conjugated to the repetitive peptide. Long-term CD3+CD4+CD8-TCR alpha beta+ T cell lines and clones were derived from both strains of mice. These lines and clones, that proliferated in an MHC-restricted fashion, did not recognize peptides from the homologous region of another murine malaria parasite, P. berghei. About 50% of these clones produced detectable amounts of IFN-gamma and IL-2, whereas the remaining produced IL-4, IL-5, and IL-6. In preliminary experiments, some of these clones specifically inhibited P. yoelii sporozoite development in vitro and conferred protection in vivo in passive transfer experiments. These findings show that heterogenous T cell populations are activated in mice upon immunization with a short peptide from the P. yoelii CS protein and that some of these cells could be active in the effector arm of the immune response against malaria sporozoites.
Keywords
Amino Acid Sequence Animals Antigens, Protozoan/chemistry/immunology CD4-Positive T-Lymphocytes/*immunology Epitopes/immunology Mice Mice, Inbred BALB C Mice, Inbred C3H Mice, Inbred C57BL Molecular Sequence Data Peptide Fragments/immunology Plasmodium berghei/*immunology Plasmodium yoelii/*immunology *Protozoan Proteins
Pubmed
Web of science
Create date
24/01/2008 14:55
Last modification date
20/08/2019 16:08
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