Expression of the vaccinia virus genome: analysis and mapping of mRNAs encoded within the inverted terminal repetition.

Details

Serval ID
serval:BIB_E47D36558350
Type
Article: article from journal or magazin.
Collection
Publications
Title
Expression of the vaccinia virus genome: analysis and mapping of mRNAs encoded within the inverted terminal repetition.
Journal
Cell
Author(s)
Wittek R., Cooper J.A., Barbosa E., Moss B.
ISSN
0092-8674[print], 0092-8674[linking]
Publication state
Published
Issued date
09/1980
Volume
21
Number
2
Pages
487-493
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
We have investigated the organization of transcriptional units within a 9000 bp segment of the terminally repeated region of the DNA genome of vaccinia virus, which uses its own enzyme system to synthesize mRNA within the cytoplasm of infected cells. RNA splicing, which has been demonstrated for DNA viruses that replicate within the nucleus of infected cells, does not appear to be involved in the formation of these first vaccinia virus mRNAs to be examined. Three immediate early mRNAs, approximately 1050, 600 and 1100 nucleotides long, were mapped between 3.21 and 4.24, 6.54 and 7.16, and 7.20 and 8.23 kb from the end of the genome, respectively. The direction of transcription was toward the end of the genome for the two larger mRNAs and in the opposite direction for the smallest one. Additional minor RNAs, which were larger in size, were mapped between and to the same DNA strand as the mRNAs of 1050 and 1100 nucleotides. No evidence for interrupted genes was obtained by nuclease S1 analysis after hybridization of RNA to labeled DNA. In addition, the 5' ends of the mRNAs, which were specifically labeled by in vitro capping, hybridized to DNA adjacent to the body of the message.
Keywords
DNA, Recombinant, DNA, Viral/genetics, Genes, Viral, Nucleic Acid Hybridization, RNA, Messenger/genetics, RNA, Viral/genetics, Transcription, Genetic, Vaccinia virus/genetics
Pubmed
Web of science
Create date
24/01/2008 10:43
Last modification date
20/08/2019 16:08
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