A simplified radioimmunoassay for physiologically active angiotensin peptides [(1-8) octa- and (2-8) heptapeptides]

Details

Serval ID
serval:BIB_E43A72AD2042
Type
Article: article from journal or magazin.
Collection
Publications
Title
A simplified radioimmunoassay for physiologically active angiotensin peptides [(1-8) octa- and (2-8) heptapeptides]
Journal
Hormone and Metabolic Research
Author(s)
Nussberger  J., Re  R., Matsueda  G. R., Haber  E.
ISSN
0018-5043 (Print)
Publication state
Published
Issued date
11/1984
Volume
16
Number
11
Pages
606-10
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Abstract
The availability of a sensitive and highly specific rabbit antiserum and the development of a peptide-extraction method employing glass beads permitted the evolution of a rapid reliable radioimmunoassay that measures the sum of the concentration of angiotensin II and its active metabolite, angiotensin III. At a dilution of 1:32,000 the antiserum is capable of measuring 1 fmol (1 pg) of angiotensin II. Cross reactivities of this antiserum, taking angiotensin II as 1.0, are: angiotensin III, 0.75; angiotensin-(3-8) hexapeptide, 0.11; angiotensin I, 0.006; angiotensin-(1-14) tetradecapeptide, 0.0001. The recovery of angiotensin II added to hormone-free plasma was 73 +/- 2% [mean +/- standard deviation (SD), n = 20]. When 0.9 ml of plasma was extracted, the minimal concentration of angiotensin II and III that could be quantified was 4 fmol/ml. When larger volumes of plasma were extracted, sensitivity was enhanced. Plasma blanks were zero. Intra-assay variability was 7.6% SD and interassay variability was 11.7% SD. Angiotensin II and III concentration in venous plasma of normal volunteers on an ad libitum diet was 15 +/- 8 fmol/ml (mean +/- SD, range less than 4 to 35 fmol/ml). The plasma of a patient with primary aldosteronism had an unmeasurable value (less than 4 fmol/ml). Posture, converting enzyme inhibition, and renal artery stenosis resulted in expected changes of angiotensin concentration.
Keywords
Angiotensin II/*analogs & derivatives/*analysis Angiotensin III/*analysis Animals Cross Reactions Humans Immune Sera Posture Rabbits/immunology Radioimmunoassay
Pubmed
Web of science
Create date
05/03/2008 16:40
Last modification date
20/08/2019 16:07
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