Characterization of cerebral glucose dynamics in vivo with a four-state conformational model of transport at the blood-brain barrier.

Details

Serval ID
serval:BIB_E43606BD1C06
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Characterization of cerebral glucose dynamics in vivo with a four-state conformational model of transport at the blood-brain barrier.
Journal
Journal of Neurochemistry
Author(s)
Duarte J.M., Gruetter R.
ISSN
1471-4159 (Electronic)
ISSN-L
0022-3042
Publication state
Published
Issued date
2012
Volume
121
Number
3
Pages
396-406
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish. PDF type: ORIGINAL ARTICLE
Abstract
Determination of brain glucose transport kinetics in vivo at steady-state typically does not allow distinguishing apparent maximum transport rate (T(max)) from cerebral consumption rate. Using a four-state conformational model of glucose transport, we show that simultaneous dynamic measurement of brain and plasma glucose concentrations provide enough information for independent and reliable determination of the two rates. In addition, although dynamic glucose homeostasis can be described with a reversible Michaelis-Menten model, which is implicit to the large iso-inhibition constant (K(ii)) relative to physiological brain glucose content, we found that the apparent affinity constant (K(t)) was better determined with the four-state conformational model of glucose transport than with any of the other models tested. Furthermore, we confirmed the utility of the present method to determine glucose transport and consumption by analysing the modulation of both glucose transport and consumption by anaesthesia conditions that modify cerebral activity. In particular, deep thiopental anaesthesia caused a significant reduction of both T(max) and cerebral metabolic rate for glucose consumption. In conclusion, dynamic measurement of brain glucose in vivo in function of plasma glucose allows robust determination of both glucose uptake and consumption kinetics.
Keywords
Algorithms, Anesthesia, Anesthetics/pharmacology, Animals, Biological Transport, Active/physiology, Blood Glucose/metabolism, Blood-Brain Barrier/physiology, Brain Chemistry/physiology, Glucose/metabolism, Homeostasis/physiology, Kinetics, Magnetic Resonance Spectroscopy, Male, Models, Biological, Rats, Rats, Sprague-Dawley
Pubmed
Web of science
Create date
29/04/2013 9:41
Last modification date
20/08/2019 16:07
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