1H-localized broadband 13C NMR spectroscopy of the rat brain in vivo at 9.4 T.

Details

Serval ID
serval:BIB_E3EBE3D93FED
Type
Article: article from journal or magazin.
Collection
Publications
Title
1H-localized broadband 13C NMR spectroscopy of the rat brain in vivo at 9.4 T.
Journal
Magnetic Resonance in Medicine
Author(s)
Henry P.G., Tkác I., Gruetter R.
ISSN
0740-3194 (Print)
ISSN-L
0740-3194
Publication state
Published
Issued date
2003
Volume
50
Number
4
Pages
684-692
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Abstract
Localized (13)C NMR spectra were obtained from the rat brain in vivo over a broad spectral range (15-100 ppm) with minimal chemical-shift displacement error (<10%) using semi-adiabatic distortionless enhancement by polarization transfer (DEPT) combined with (1)H localization. A new gradient dephasing scheme was employed to eliminate unwanted coherences generated by DEPT when using surface coils with highly inhomogeneous B(1) fields. Excellent sensitivity was evident from the simultaneous detection of natural abundance signals for N-acetylaspartate, myo-inositol, and glutamate in the rat brain in vivo at 9.4 T. After infusion of (13)C-labeled glucose, up to 18 (13)C resonances were simultaneously measured in the rat brain, including glutamate C2, C3, C4, glutamine C2, C3, C4, aspartate C2, C3, glucose C1, C6, N-acetyl-aspartate C2, C3, C6, as well as GABA C2, lactate C3, and alanine C3. (13)C-(13)C multiplets corresponding to multiply labeled compounds were clearly observed, suggesting that extensive isotopomer analysis is possible in vivo. This unprecedented amount of information will be useful for metabolic modeling studies aimed at understanding brain energy metabolism and neurotransmission in the rodent brain.
Keywords
Animals, Brain/metabolism, Brain Chemistry, Carbon Isotopes, Glucose/diagnostic use, Magnetic Resonance Spectroscopy/diagnostic use, Magnetic Resonance Spectroscopy/methods, Male, Rats, Rats, Sprague-Dawley
Pubmed
Open Access
Yes
Create date
04/08/2010 15:28
Last modification date
20/08/2019 16:07
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