Alternative 5' end of the bcr-abl transcript in chronic myelogenous leukemia

Details

Serval ID
serval:BIB_E3B3A45D8DF4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Alternative 5' end of the bcr-abl transcript in chronic myelogenous leukemia
Journal
Oncogene
Author(s)
Romero  P., Beran  M., Shtalrid  M., Andersson  B., Talpaz  M., Blick  M.
ISSN
0950-9232 (Print)
Publication state
Published
Issued date
01/1989
Volume
4
Number
1
Pages
93-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan
Abstract
Philadelphia chromosome positive acute lymphocytic leukemia and chronic myelogenous leukemia are strongly associated with two distinct forms of bcr-abl chimeric protein, known as P190 and P210, respectively. By studying cDNA clones obtained from the cell line KBM-5, we identified two new bcr-abl transcripts. These are formed by alternative splicing of at least two exons to the known bcr exon 2. One novel transcript can encode a protein kinase of approximately 190 kd, while the other can direct the synthesis of a larger protein whose amino terminus remains to be defined. The alternative exons can be spliced also to the two normal bcr transcripts, reflecting the activation of a cryptic promoter. These messages were present at low abundance in two cases of blastic crisis but were not detected in the chronic phase. It is conceivable that the proteins encoded by the new bcr-abl mRNAs are involved in the transformation to the acute phase in some cases of chronic myelogenous leukemia.
Keywords
Amino Acid Sequence Base Sequence Cell Line Chimera *Cloning, Molecular Dna DNA Probes Exons Gene Rearrangement Humans Immunoblotting Leukemia, Myeloid, Chronic/*genetics Molecular Sequence Data *Oncogenes RNA, Messenger/*ultrastructure RNA, Neoplasm/*ultrastructure
Pubmed
Web of science
Create date
28/01/2008 11:28
Last modification date
20/08/2019 16:07
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