Destabilization induced by electropermeabilization analyzed by atomic force microscopy

Details

Serval ID
serval:BIB_E33410D921F8
Type
Article: article from journal or magazin.
Collection
Publications
Title
Destabilization induced by electropermeabilization analyzed by atomic force microscopy
Journal
Biochimica et Biophysica Acta (BBA) - Biomembranes
Author(s)
Chopinet Louise, Roduit Charles, Rols Marie-Pierre, Dague Etienne
ISSN
0005-2736
ISSN-L
1879-2642
Publication state
Published
Issued date
09/2013
Peer-reviewed
Oui
Volume
1828
Number
9
Pages
2223-2229
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
Electropermeabilization is a physical method that uses electric field pulses to deliver molecules into cells and tissues. Despite its increasing interest in clinics, little is known about plasma membrane destabilization process occurring during electropermeabilization. In this work, we took advantage of atomic force microscopy to directly visualize the consequences of electropermeabilization in terms of membrane reorganization and to locally measure the membrane elasticity. We visualized transient rippling of membrane surface and measured a decrease in membrane elasticity by 40%. Our results obtained both on fixed and living CHO cells give evidence of an inner effect affecting the entire cell surface that may be related to cytoskeleton destabilization. Thus, AFM appears as a useful tool to investigate basic process of electroporation on living cells in absence of any staining or cell preparation.
Keywords
Animals, CHO Cells, Cell Membrane/chemistry, Cell Membrane Permeability, Cricetinae, Cytoskeleton/chemistry, Elasticity, Electroporation, Microscopy, Atomic Force, Biophysics, Cell Biology, Biochemistry
Pubmed
Web of science
Open Access
Yes
Create date
30/08/2013 16:02
Last modification date
20/08/2019 16:07
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