Male-specific fruitless isoforms target neurodevelopmental genes to specify a sexually dimorphic nervous system.

Details

Serval ID
serval:BIB_E3227A549E40
Type
Article: article from journal or magazin.
Collection
Publications
Title
Male-specific fruitless isoforms target neurodevelopmental genes to specify a sexually dimorphic nervous system.
Journal
Current Biology
Author(s)
Neville M.C., Nojima T., Ashley E., Parker D.J., Walker J., Southall T., Van de Sande B., Marques A.C., Fischer B., Brand A.H., Russell S., Ritchie M.G., Aerts S., Goodwin S.F.
ISSN
1879-0445 (Electronic)
ISSN-L
0960-9822
Publication state
Published
Issued date
2014
Volume
24
Number
3
Pages
229-241
Language
english
Abstract
BACKGROUND: In Drosophila, male courtship behavior is regulated in large part by the gene fruitless (fru). fru encodes a set of putative transcription factors that promote male sexual behavior by controlling the development of sexually dimorphic neuronal circuitry. Little is known about how Fru proteins function at the level of transcriptional regulation or the role that isoform diversity plays in the formation of a male-specific nervous system.
RESULTS: To characterize the roles of sex-specific Fru isoforms in specifying male behavior, we generated novel isoform-specific mutants and used a genomic approach to identify direct Fru isoform targets during development. We demonstrate that all Fru isoforms directly target genes involved in the development of the nervous system, with individual isoforms exhibiting unique binding specificities. We observe that fru behavioral phenotypes are specified by either a single isoform or a combination of isoforms. Finally, we illustrate the utility of these data for the identification of novel sexually dimorphic genomic enhancers and novel downstream regulators of male sexual behavior.
CONCLUSIONS: These findings suggest that Fru isoform diversity facilitates both redundancy and specificity in gene expression, and that the regulation of neuronal developmental genes may be the most ancient and conserved role of fru in the specification of a male-specific nervous system.
Keywords
Animals, Base Sequence, Central Nervous System/metabolism, Drosophila Proteins/genetics, Drosophila melanogaster/genetics, Drosophila melanogaster/metabolism, Gene Expression Regulation, Developmental/genetics, Gene Knockout Techniques, Male, Molecular Sequence Data, Nerve Tissue Proteins/genetics, Neurogenesis/genetics, Protein Isoforms/genetics, Sex Characteristics, Sexual Behavior, Animal/physiology, Transcription Factors/genetics
Pubmed
Web of science
Open Access
Yes
Create date
27/10/2014 14:44
Last modification date
20/08/2019 16:06
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