The CD8 beta polypeptide is required for the recognition of an altered peptide ligand as an agonist.

Details

Serval ID
serval:BIB_E31ED68D6052
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The CD8 beta polypeptide is required for the recognition of an altered peptide ligand as an agonist.
Journal
European journal of immunology
Author(s)
Renard V., Delon J., Luescher I.F., Malissen B., Vivier E., Trautmann A.
ISSN
0014-2980
Publication state
Published
Issued date
1996
Peer-reviewed
Oui
Volume
26
Number
12
Pages
2999-3007
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
T cell activation is triggered by the specific recognition of cognate peptides presented by MHC molecules. Altered peptide ligands are analogs of cognate peptides which have a high affinity for MHC molecules. Some of them induce complete T cell responses, i.e. they act as agonists, whereas others behave as partial agonists or even as antagonists. Here, we analyzed both early (intracellular Ca2+ mobilization), and late (interleukin-2 production) signal transduction events induced by a cognate peptide or a corresponding altered peptide ligand using T cell hybridomas expressing or not the CD8 alpha and beta chains. With a video imaging system, we showed that the intracellular Ca2+ response to an altered peptide ligand induces the appearance of a characteristic sustained intracellular Ca2+ concentration gradient which can be detected shortly after T cell interaction with antigen-presenting cells. We also provide evidence that the same altered peptide ligand can be seen either as an agonist or a partial agonist, depending on the presence of CD8beta in the CD8 co-receptor dimers expressed at the T cell surface.
Keywords
Animals, Antigen Presentation/drug effects, Antigens, CD8/pharmacology, Calcium/metabolism, Cell Line, Interleukin-2/biosynthesis, L Cells (Cell Line), Ligands, Mice, Peptides/agonists, Peptides/immunology, Signal Transduction/drug effects, T-Lymphocytes, Cytotoxic/immunology, T-Lymphocytes, Cytotoxic/metabolism
Pubmed
Web of science
Create date
28/01/2008 11:20
Last modification date
20/08/2019 16:06
Usage data