Linkage between rheumatoid arthritis susceptibility and the presence of HLA-DR4 and DR beta allelic third hypervariable region sequences in southern Chinese persons

Details

Serval ID
serval:BIB_E2DD92CAC4FC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Linkage between rheumatoid arthritis susceptibility and the presence of HLA-DR4 and DR beta allelic third hypervariable region sequences in southern Chinese persons
Journal
Arthritis and Rheumatism
Author(s)
Seglias  J., Li  E. K., Cohen  M. G., Wong  R. W., Potter  P. K., So  A. K.
ISSN
0004-3591 (Print)
Publication state
Published
Issued date
02/1992
Volume
35
Number
2
Pages
163-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Abstract
OBJECTIVE. To analyze HLA-DR and DQ associations with rheumatoid arthritis (RA) in patients from southern China. METHODS. In 66 patients and 45 controls, restriction fragment length polymorphism studies were performed using DRB, DQA, and DQB probes, and DRB allele-specific typing of polymerase chain reaction-amplified DRB DNA. RESULTS. The frequency of HLA-DR4 was significantly increased among RA patients (42.4% versus 17.8%). Increased frequencies of the DQA3 allele (77.8% versus 48.9%) and the DQB1*0302 allele (71.0% versus 46.3%), which are in linkage disequilibrium with DR4, were also found. Oligonucleotide typing showed that the amino acid sequence LLEQRRAA, spanning amino acid positions 67-74 of the DR beta molecule, was found in 19 of 49 patients and 5 of 32 controls. The main DR4 allelic subtypes found in the population were DRB1*0404 and DRB1*0405, both of which carried the sequence. There was no difference in subtype distribution between patients and controls. CONCLUSION. Chinese RA patients have an increased frequency of HLA-DR4 alleles which possess the same DRB third allelic hypervariable sequence shown to be associated with susceptibility in Caucasian RA patients.
Keywords
*Alleles Amino Acid Sequence Arthritis, Rheumatoid/*ethnology/genetics/*immunology China Disease Susceptibility Gene Amplification HLA-DR Antigens/*genetics Humans Hybridization, Genetic Linkage (Genetics) Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Restriction Fragment Length
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 8:38
Last modification date
20/08/2019 16:06
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