The importance of cell contact for the proliferation of neuroblasts in culture and its stimulation by meningeal extract.

Details

Serval ID
serval:BIB_E252A88682F1
Type
Article: article from journal or magazin.
Collection
Publications
Title
The importance of cell contact for the proliferation of neuroblasts in culture and its stimulation by meningeal extract.
Journal
Neurochemical research
Author(s)
Barakat I., Sensenbrenner M., Vincendon G.
ISSN
0364-3190
Publication state
Published
Issued date
1982
Peer-reviewed
Oui
Volume
7
Number
3
Pages
287-300
Language
english
Abstract
The influence of cell density and cell contacts on the proliferation of neuroblasts in culture and its stimulation by meningeal extract were investigated. Dissociated brain cells from 6-day-old chick embryos were cultured under 3 different culture conditions to obtain dense or sparse brain cell cultures, as well as cultures of isolated neuronal cell. The proliferation fo neuroblasts, shown by morphological observations, cell counts, determinations of DNA content and measurements of [3H]thymidine incorporation, was found to be the highest in cultures where cell density and cellular contacts were greatest. The addition of meningeal extract stimulated the multiplication of neuroblasts only in cultures where the cells were in closer with each other. The results suggested, therefore, that cell density and cell-cell interactions are of importance and favored neuroblast proliferation.
Keywords
Animals, Cell Communication, Cell Count, Cell Division, Cells, Cultured, Chick Embryo, Collagen, DNA, Meninges, Neurons, Polylysine, Thymidine, Tissue Extracts
Pubmed
Web of science
Create date
30/03/2009 9:34
Last modification date
20/08/2019 16:06
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