Searching the expressed sequence tag (EST) databases: panning for genes.
Details
Serval ID
serval:BIB_E2133358ABF7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Searching the expressed sequence tag (EST) databases: panning for genes.
Journal
Briefings in Bioinformatics
ISSN
1467-5463[print], 1467-5463[linking]
Publication state
Published
Issued date
02/2000
Volume
1
Number
1
Pages
76-92
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
The genomes of living organisms contain many elements, including genes coding for proteins. The portions of the genes expressed as mature mRNA, collectively known as the transcriptome, represent only a small part of the genome. The expressed sequence tag (EST) databases contain an increasingly large part of the transcriptome of many species. For this reason, these databases are probably the most abundant source of new coding sequences available today. However, the raw data deposited in the EST databases are to a large extent unorganised, unannotated, redundant and of relatively low quality. This paper reviews some of the characteristics of the EST data, and the methods that can be used to find novel protein sequences within them. It also documents a collection of databases, software and web sites that can be useful to biologists interested in mining the EST databases over the Internet, or in establishing a local environment for such analyses.
Keywords
Algorithms, Animals, Computational Biology/methods, Contig Mapping, DNA, Complementary/genetics, Databases, Factual, Expressed Sequence Tags, Frameshift Mutation, Genes, Genome, Humans, Internet, RNA, Messenger/genetics, Software, Transcription, Genetic
Pubmed
Create date
24/01/2008 16:39
Last modification date
20/08/2019 17:06