Extraction of skin test activity from Coccidioides immitis mycelia by water, perchloric acid, and aqueous phenol extraction.

Details

Serval ID
serval:BIB_E16FEDE3275E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Extraction of skin test activity from Coccidioides immitis mycelia by water, perchloric acid, and aqueous phenol extraction.
Journal
Infection and Immunity
Author(s)
Wheat R.W., Su Chung K.S., Ornellas E.P., Scheer E.R.
ISSN
0019-9567 (Print)
ISSN-L
0019-9567
Publication state
Published
Issued date
1978
Peer-reviewed
Oui
Volume
19
Number
1
Pages
152-159
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, U.S. Gov't, P.H.S. Publication Status: ppublish
Abstract
Water, perchloric acid extracts, and fractions of partially defatted whole mycelia of Coccidioides immitis contained delayed skin test activity when tested in C. immitis-infected guinea pits. Aqueous phenol extraction of these fractions resulted in partitioning of activity between aqueous-soluble and phenol-soluble fractions; activity was found to be water soluble after removal of phenol by extensive dialysis. Highest specific activity skin test antigen was invariably found in the phenol-soluble phase, water-soluble fraction. Material of equivalent activity could also be extracted directly from the defatted mycelia. Skin test active fractions contained glucose, mannose, 3-O-methylmannose, glucosamine, and amino acids.
Keywords
Animals, Antigens, Fungal/analysis, Antigens, Fungal/isolation & purification, Coccidioides/immunology, Glucosamine/analysis, Glucose/analysis, Guinea Pigs, Mannose/analysis, Perchloric Acid, Phenols, Skin Tests, Solubility, Water
Pubmed
Web of science
Create date
29/01/2008 16:20
Last modification date
20/08/2019 17:05
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