Dietary protein modifies oxidized cholesterol-induced alterations of linoleic acid and cholesterol metabolism in rats

Details

Serval ID
serval:BIB_E1515C058B26
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Dietary protein modifies oxidized cholesterol-induced alterations of linoleic acid and cholesterol metabolism in rats
Journal
Journal of Nutrition
Author(s)
Osada K., Kodama T., Minehira K., Yamada K., Sugano M.
ISSN
0022-3166
Publication state
Published
Issued date
1996
Peer-reviewed
Oui
Volume
126
Number
6
Pages
1635-43
Language
english
Abstract
Effects of dietary protein on oxidized cholesterol-induced alterations in linoleic acid and cholesterol metabolism were studied in 4-wk-old male Sprague-Dawley rats, using casein and soybean protein as dietary protein sources. The rats were fed one of the two proteins in cholesterol-free, 0.3% cholesterol or 0.3% oxidized cholesterol mixture diets using a pair-feeding protocol for 3 wk. In the soybean protein-fed group, rats fed oxidized cholesterol did not have lower activity of liver microsomal delta6 desaturase, the rate-limiting enzyme in the metabolism of linoleic acid to arachidonic acid, compared with rats fed cholesterol-free diet, whereas in the casein-fed group the desaturase activity was significantly greater in rats fed oxidized cholesterol than in those fed cholesterol-free diet. This was in contrast to a significant reduction in liver microsomal delta6 desaturase activity by cholesterol, irrespective of protein source. In general, these changes were reflected in the desaturation indices of liver phospholipids. Furthermore, soybean protein significantly increased the fecal excretion of neutral and acidic steroids and tended to reduce (P = 0.082) the accumulation of oxidized cholesterols in the liver. Thus, soybean protein partly modified some of the undesirable effects of oxidized cholesterol through its hypocholesterolemic effect and possibly through the modulation of hepatic delta6 desaturase activity.
Keywords
AnimalsCaseins/administration & dosage/pharmacologyCholesterol/*metabolismCholesterol, Dietary/administration & dosage/*pharmacologyDietary Proteins/administration & dosage/*pharmacologyFatty Acid Desaturases/metabolismLinoleic AcidLinoleic Acids/*metabolismLinoleoyl-CoA DesaturaseMaleMicrosomes, Liver/enzymologyOxidation-ReductionRatsRats, Sprague-DawleySoybean ProteinsVegetable Proteins/administration & dosage/pharmacology
Pubmed
Web of science
Create date
20/02/2009 13:43
Last modification date
20/08/2019 17:05
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