Serological diagnosis of experimental Enterococcus faecalis endocarditis

Details

Serval ID
serval:BIB_E147B1B5F103
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Serological diagnosis of experimental Enterococcus faecalis endocarditis
Journal
APMIS
Author(s)
Kjerulf  A., Espersen  F., Gutschik  E., Majcherczyk  P. A., Hougen  H. P., Rygaard  J., Hoiby  N.
ISSN
0903-4641 (Print)
Publication state
Published
Issued date
10/1998
Volume
106
Number
10
Pages
997-1008
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Abstract
A modified rat model of endocarditis with catheterization for 2 days was established in female Lewis rats using different inocula of Enterococcus faecalis (strain no. EF 19) in order to measure IgG antibodies in serum during the course of infection. Increasing the inocula intravenously resulted in an increase in the CFU/g vegetation and the CFU/g spleen, the ID50 being about 10 CFU/ml and the ID90 about 1x10(2) CFU/ml. The lowest bacterial inoculum infecting 100% of the rats was 3x10(3) CFU/ml, and for further investigations we used this inoculum size. Rats were sacrificed on day 2, 5, 7, 9, 11 and 28 after infection. The CFU/g vegetation and the CFU/g spleen increased until day 7 and then decreased. Serum samples were collected from 129 rats at different times after challenge. Three different ELISA systems were established to measure the IgG antibody responses: E. faecalis sonicate ELISA (a pool of four sonicates of strain no. EF 10, EF 11, EF 19 and EF 48), E. faecalis whole cell ELISA (strain no. EF 19) and E. faecalis purified cell wall ELISA (strain no. EF 19). An IgG antibody response was detected already on day 2, and except for a minor decrease on day 6/7 the antibody response continued to increase until day 14 (whole cell ELISA and sonicate ELISA) and day 21 (purified cell wall ELISA) when a plateau was reached. Significant increases in IgG antibody responses (p<0.05) were found between groups of rats from days 0-2, 2-8/9 and 8/9-14 in the E. faecalis whole cell and sonicate ELISAs and from days 0-2, 2-10/11 and 10/11-21 in the E. faecalis purified cell wall ELISA. In conclusion, we established a model of endocarditis in rats with catheterization for 2 days and were able to demonstrate an increase in IgG antibodies during the course of infection.
Keywords
Animals Blotting, Western Cell Fractionation Cell Wall/chemistry/immunology Cross Reactions Disease Models, Animal Electrophoresis, Polyacrylamide Gel Endocarditis, Bacterial/*diagnosis/immunology/pathology Enterococcus faecalis/immunology/*isolation & purification Enzyme-Linked Immunosorbent Assay Female Gram-Positive Bacterial Infections/*diagnosis/immunology/pathology Heart Valves/microbiology/pathology Rats Rats, Inbred Lew
Pubmed
Web of science
Create date
24/01/2008 13:54
Last modification date
20/08/2019 16:05
Usage data