T cell receptor selection by and recognition of two class I major histocompatibility complex-restricted antigenic peptides that differ at a single position.

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Version: Final published version
License: CC BY-NC-SA 4.0
Serval ID
serval:BIB_E11D349A25BF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
T cell receptor selection by and recognition of two class I major histocompatibility complex-restricted antigenic peptides that differ at a single position.
Journal
Journal of Experimental Medicine
Author(s)
Casanova J.L., Martinon F., Gournier H., Barra C., Pannetier C., Regnault A., Kourilsky P., Cerottini J.C., Maryanski J.L.
ISSN
0022-1007 (Print)
ISSN-L
0022-1007
Publication state
Published
Issued date
1993
Volume
177
Number
3
Pages
811-820
Language
english
Abstract
Peptides derived from HLA-Cw3 and HLA-A24 within region 170-179 differ by a single substitution, at position 173, and are both presented by the class I major histocompatibility complex molecule H-2Kd for recognition by murine cytolytic T lymphocytes (CTLs). As a first approach to understand the way T cell receptors (TCRs) intact with the HLA peptides, we have analyzed the TCR selection by, and recognition of, the two HLA antigenic sites. First, we have compared the TCR repertoires selected by HLA-Cw3 and HLA-A24, not only by sequencing the TCRs carried by CTL clones isolated and grown in vitro, but also by analyzing the TCRs expressed in vivo by peritoneal exudate lymphocytes from immune animals. Second, we have compared the TCR crossrecognition of HLA-A24 by CTLs selected by HLA-Cw3 with that of HLA-Cw3 by CTLs selected by HLA-A24. The combined analysis of TCR selection by and recognition of these two related HLA antigenic sites provides evidence that the TCR beta junctional regions interact with the amino-terminal part of the HLA peptides.
Keywords
Amino Acid Sequence, Animals, Base Sequence, Cell Line, DNA/analysis, DNA/genetics, HLA-A Antigens/analysis, HLA-A Antigens/genetics, HLA-C Antigens/analysis, HLA-C Antigens/genetics, Histocompatibility Antigens Class I/analysis, Histocompatibility Antigens Class I/genetics, Major Histocompatibility Complex/genetics, Major Histocompatibility Complex/immunology, Mice, Molecular Sequence Data, Peritoneum/cytology, Polymerase Chain Reaction, Receptors, Antigen, T-Cell/analysis, Receptors, Antigen, T-Cell/genetics, Receptors, Antigen, T-Cell, alpha-beta/analysis, Receptors, Antigen, T-Cell, alpha-beta/genetics, T-Lymphocytes/chemistry, T-Lymphocytes/immunology
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 12:14
Last modification date
20/08/2019 17:05
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