Cloning of yeast transfer RNA genes in Escherichia coli
Details
Serval ID
serval:BIB_E1135ED8B3BC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cloning of yeast transfer RNA genes in Escherichia coli
Journal
Science
ISSN
0036-8075 (Print)
Publication state
Published
Issued date
04/1977
Volume
196
Number
4286
Pages
205-8
Notes
Journal Article
Research Support, U.S. Gov't, P.H.S. --- Old month value: Apr 8
Research Support, U.S. Gov't, P.H.S. --- Old month value: Apr 8
Abstract
Four thousand Escherichia coli clones containing yeast DNA inserted into the plasmid pBR313 have been isolated. Of these, 175 clones were identified as carrying yeast transfer RNA genes. The initial analysis of the inserted transfer RNA genes via the colony hybridization technique with individual radioactive transfer RNA species is reported. The data indicate that yeast transfer RNA genes are not highly clustered, although some clustering exists. In addition, it was observed that the reiteration number of different transfer RNA genes may vary extensively.
Keywords
Chromosome Mapping
DNA Restriction Enzymes
DNA, Bacterial/analysis
DNA, Recombinant/*analysis
Escherichia coli
*Genes
Molecular Weight
Nucleic Acid Hybridization
Plasmids
*RNA, Transfer
Saccharomyces cerevisiae/*analysis
Pubmed
Web of science
Create date
25/01/2008 17:17
Last modification date
20/08/2019 17:05