Differential expression of laminin-5 subunits and integrin receptors in human colorectal neoplasia

Details

Serval ID
serval:BIB_E10030DD7993
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Differential expression of laminin-5 subunits and integrin receptors in human colorectal neoplasia
Journal
Journal of Pathology
Author(s)
Sordat  I., Bosman  F. T., Dorta  G., Rousselle  P., Aberdam  D., Blum  A. L., Sordat  B.
ISSN
0022-3417 (Print)
Publication state
Published
Issued date
05/1998
Volume
185
Number
1
Pages
44-52
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Abstract
Cell-matrix interactions contribute to regulating the adhesion, growth, migration, and differentiation of epithelial intestinal cells. Alterations in matrix components and their cellular receptors have been found in tumours but their specific roles remain unclear. The tissue patterns of laminin-5 and alpha 3, beta 3 and gamma 2 subunits, as well as those of the alpha 3, alpha 6, beta 1, and beta 4 integrin chains, were determined by immunofluorescence on frozen sections of 12 colorectal mucosal samples from four patients, 15 adenomas, 29 adenocarcinomas, and eight metastases. Distinct patterns of laminin-5 and integrin expression were found along the mucosa-adenoma, and adenoma-carcinoma transitions. Expression of basement membrane laminin-5 and subunits was continuous and gradient-like in normal mucosa, enhanced at the periphery of adenomas, and discontinuous in places in carcinomas and metastases. Decrease of the alpha 3 integrin chain was found in adenomas, together with that of alpha 6 and beta 4 chains in carcinomas. A subpopulation of carcinoma cells dissociating (budding) from neoplastic tubules was found to accumulate the laminin-5 beta 3 gamma 2 heterodimer in the cytoplasm, with progressive loss of surface integrin expression. These results suggest that in colorectal cancer, an abnormal expression of laminin-5 subunits and integrin chains may identify a subset of carcinoma cells prone to invade focally and to contribute to disease aggressiveness.
Keywords
Adenocarcinoma/metabolism Adenoma/metabolism Cell Adhesion Molecules/*metabolism Colon/metabolism Colorectal Neoplasms/*metabolism/pathology Fluorescent Antibody Technique, Indirect Humans Integrins/*metabolism Intestinal Mucosa/metabolism Liver Neoplasms/metabolism/secondary Microscopy, Confocal Neoplasm Proteins/*metabolism
Pubmed
Web of science
Create date
25/01/2008 16:48
Last modification date
20/08/2019 17:05
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