Detection of rare circulating human colon tumor cells in a nude mouse xenograft model

Details

Serval ID
serval:BIB_E05383C04840
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Detection of rare circulating human colon tumor cells in a nude mouse xenograft model
Journal
Cancer Letters
Author(s)
Pages  J. C., Sordat  B., Bautista  D., Costa  J., Benhattar  J.
ISSN
0304-3835 (Print)
Publication state
Published
Issued date
1996
Volume
106
Number
1
Pages
139-144
Notes
PT - Journal Article PT - Research Support, Non-U.S. Gov't
Abstract
A highly sensitive allele specific polymerase chain reaction was developed and applied to the detection of K-ras mutation in human colon tumor cells both in the blood and in tissues. An experimental model of human colon carcinoma cells, carrying a GAT mutation in the 12th codon of the K-ras gene, and grafted into nude mice has been selected for evaluating the occurrence of cells in the course of disseminating into the host. We have found tumor cells circulating in the blood starting 37 days following subcutaneous primary implantation. Occasional micrometastatic deposits could be detected in lymph node draining the xenograft, but no tumor cells were found in lungs and mediastinum. In this experimental model, our results indicate that the mere presence of tumor cells in the blood does not imply the full accomplishment of the multi-step metastatic process
Keywords
Animals/Base Sequence/Codon/genetics/Colonic Neoplasms/blood/Pathology/DNA Primers/Female/Genes,ras/Humans/Lymphatic Metastasis/Mice/Mice,Nude/Neoplasm Metastasis/Point Mutation/Polymerase Chain Reaction/methods/Sensitivity and Specificity/Transplantation,Heterologous
Pubmed
Web of science
Create date
29/01/2008 19:34
Last modification date
20/08/2019 17:04
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