Exploring neural cell dynamics with digital holographic microscopy.

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State: Public
Version: author
Serval ID
serval:BIB_DFCF40B4AA1A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Exploring neural cell dynamics with digital holographic microscopy.
Journal
Annual Review of Biomedical Engineering
Author(s)
Marquet P., Depeursinge C., Magistretti P.J.
ISSN
1545-4274 (Electronic)
ISSN-L
1523-9829
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
15
Pages
407-431
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
In this review, we summarize how the new concept of digital optics applied to the field of holographic microscopy has allowed the development of a reliable and flexible digital holographic quantitative phase microscopy (DH-QPM) technique at the nanoscale particularly suitable for cell imaging. Particular emphasis is placed on the original biological information provided by the quantitative phase signal. We present the most relevant DH-QPM applications in the field of cell biology, including automated cell counts, recognition, classification, three-dimensional tracking, discrimination between physiological and pathophysiological states, and the study of cell membrane fluctuations at the nanoscale. In the last part, original results show how DH-QPM can address two important issues in the field of neurobiology, namely, multiple-site optical recording of neuronal activity and noninvasive visualization of dendritic spine dynamics resulting from a full digital holographic microscopy tomographic approach.
Pubmed
Web of science
Create date
06/08/2013 14:40
Last modification date
20/08/2019 17:04
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