Short term Candida albicans colonization reduces Pseudomonas aeruginosa-related lung injury and bacterial burden in a murine model

Details

Serval ID
serval:BIB_DF7A6C114806
Type
Article: article from journal or magazin.
Collection
Publications
Title
Short term Candida albicans colonization reduces Pseudomonas aeruginosa-related lung injury and bacterial burden in a murine model
Journal
Crit Care
Author(s)
Ader F., Jawhara S., Nseir S., Kipnis E., Faure K., Vuotto F., Chemani C., Sendid B., Poulain D., Guery B.
ISSN
1466-609X (Electronic)
ISSN-L
1364-8535
Publication state
Published
Issued date
2011
Volume
15
Number
3
Pages
R150
Language
english
Notes
Ader, Florence
Jawhara, Samir
Nseir, Saad
Kipnis, Eric
Faure, Karine
Vuotto, Fanny
Chemani, Chanez
Sendid, Boualem
Poulain, Daniel
Guery, Benoit
eng
England
Crit Care. 2011 Jun 20;15(3):R150. doi: 10.1186/cc10276.
Abstract
INTRODUCTION: Pseudomonas aeruginosa is a frequent cause of ventilator-acquired pneumonia (VAP). Candida tracheobronchial colonization is associated with higher rates of VAP related to P. aeruginosa. This study was designed to investigate whether prior short term Candida albicans airway colonization modulates the pathogenicity of P. aeruginosa in a murine model of pneumonia and to evaluate the effect of fungicidal drug caspofungin. METHODS: BALB/c mice received a single or a combined intratracheal administration of C. albicans (1 x 10(5) CFU/mouse) and P. aeruginosa (1 x 10(7) CFU/mouse) at time 0 (T0) upon C. albicans colonization, and Day 2. To evaluate the effect of antifungal therapy, mice received caspofungin intraperitoneally daily, either from T0 or from Day 1 post-colonization. After sacrifice at Day 4, lungs were analyzed for histological scoring, measurement of endothelial injury, and quantification of live P. aeruginosa and C. albicans. Blood samples were cultured for dissemination. RESULTS: A significant decrease in lung endothelial permeability, the amount of P. aeruginosa, and bronchiole inflammation was observed in case of prior C. albicans colonization. Mortality rate and bacterial dissemination were unchanged by prior C. albicans colonization. Caspofungin treatment from T0 (not from Day 1) increased their levels of endothelial permeability and lung P. aeruginosa load similarly to mice receiving P. aeruginosa alone. CONCLUSIONS: P. aeruginosa-induced lung injury is reduced when preceded by short term C. albicans airway colonization. Antifungal drug caspofungin reverses that effect when used from T0 and not from Day 1.
Keywords
Animals, Bacterial Load/methods/*physiology, Candida albicans/*growth & development, Colony Count, Microbial/methods, *Disease Models, Animal, Lung Injury/*microbiology/*prevention & control, Mice, Mice, Inbred BALB C, Pseudomonas Infections/physiopathology/*prevention & control, Pseudomonas aeruginosa/*growth & development/isolation & purification, Random Allocation, Time Factors
Pubmed
Create date
29/04/2021 10:59
Last modification date
30/04/2021 6:38
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