Effects of gonadectomy and sex hormone therapy on the endotoxin-stimulated hypothalamo-pituitary-adrenal axis: evidence for a neuroendocrine-immunological sexual dimorphism

Details

Serval ID
serval:BIB_DF49F1B36CE9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Effects of gonadectomy and sex hormone therapy on the endotoxin-stimulated hypothalamo-pituitary-adrenal axis: evidence for a neuroendocrine-immunological sexual dimorphism
Journal
Endocrinology
Author(s)
Spinedi  E., Suescun  M. O., Hadid  R., Daneva  T., Gaillard  R. C.
ISSN
0013-7227 (Print)
Publication state
Published
Issued date
11/1992
Volume
131
Number
5
Pages
2430-6
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Abstract
Bacterial lipopolysaccharide (LPS) stimulates the hypothalamo-pituitary-adrenal axis by a mechanism involving the release of cytokines, which activate the CRH-ACTH system and, as a result, increase glucocorticoid secretion. In the present study we investigated the possibility that endogenous sex hormones modulate the in vivo endotoxin-stimulated adrenal and immune responses in adult BALB/c mice. In preliminary experiments we determined that the maximal glucocorticoid release in response to LPS (50 micrograms, ip) administration was reached 2 h after treatment. The endotoxin effect on the adrenal and immune responses was then tested in male, randomly cycling female, 20-day-gonadectomized and 20-day-gonadectomized mice treated with either testosterone or estradiol. In addition, in vitro experiments were performed to determine whether 1) LPS exerts any direct effect on basal and ACTH-stimulated corticosterone release, and 2) adrenal function is influenced by bilateral gonadectomy and sex steroid therapy. Our results indicate that 1) randomly cycling female mice have significantly more pronounced corticosterone secretion than males 2 h after endotoxin injection, although the tumor necrosis factor responses were similar; 2) the response of the hypothalamo-pituitary-adrenal axis to endotoxin stimulation in female mice was invariable throughout the different stages of the normal estrous cycle; 3) gonadectomy leads to enhanced (P < 0.05) adrenal and immune responses to LPS stimulation compared to the responses in shams; 4) the endotoxin-elicited adrenal and immune overresponses observed in gonadectomized mice are reversed by testosterone treatment, regardless of sex; 5) LPS does not directly modify spontaneous and ACTH-stimulated adrenal corticosterone secretion; and 6) gonadectomy alone or combined with sex steroid therapy does not increase the in vitro adrenal response to ACTH stimulation. Our findings further suggest an evident neuroendocrine-immunological sexual dimorphism during the acute phase of inflammatory processes.
Keywords
Adrenocorticotropic Hormone/pharmacology Animals Corticosterone/blood Endotoxins/*pharmacology Estradiol/*pharmacology Estrus/physiology Female Glucocorticoids/classification Hypothalamo-Hypophyseal System/*drug effects Immune System/drug effects/physiology Lipopolysaccharides/pharmacology Male Mice Mice, Inbred BALB C Neurosecretory Systems/drug effects/physiology *Orchiectomy *Ovariectomy Pituitary-Adrenal System/*drug effects *Sex Characteristics Testosterone/*pharmacology Time Factors Tumor Necrosis Factor-alpha/analysis
Pubmed
Web of science
Create date
15/02/2008 16:57
Last modification date
20/08/2019 16:03
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