Spatiotemporal gradients of kainate-sensitivity in the developing chicken retina.

Details

Serval ID
serval:BIB_DF3A18FA679B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Spatiotemporal gradients of kainate-sensitivity in the developing chicken retina.
Journal
Journal of Comparative Neurology
Author(s)
Catsicas S., Clarke P.G.H.
ISSN
0021-9967[print], 0021-9967[linking]
Publication state
Published
Issued date
1987
Volume
262
Number
4
Pages
512-522
Language
english
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Aug 22
Abstract
We have studied the age-dependence of the effects of kainate (KA) on the chick retina as a prelude to the accompanying paper on the effects of target-removal on the isthmo-optic nucleus. KA was injected into the eyes of chick embryos and chicks at different ages, and the retinas were fixed a few hours or several days later. The former group of retinas was scanned for pyknotic cells. The earliest age at which KA caused pyknosis was embryonic day 10 (E10), when pyknotic cells appeared in a ventrotemporal patch in the amacrine sublayer near the fundus. Over the next two days the sensitive region expanded tangentially, reaching the periphery first temporally, then nasally. Only after E12 did the KA cause pyknotic cells to occur also in the bipolar sublayer, where the sensitivity spread in the same spatiotemporal sequence as the initial wave, but two days later. Cell loss was examined in embryos that survived a week or more after the KA injection. Substantial cell depletion was found in both the inner nuclear and ganglion cell layers, but only when the injection had been made after E12. With progressively later injections, the depleted zone expanded in the same spatiotemporal sequence as described above, until at E15 the injections caused depletion throughout the entire extent of the retina. The reasons for the lack of cell depletion after KA injections made before E12 are discussed. Cell counts in the ganglion cell layer and studies of anterograde transport of intravitreally injected peroxidase along the retinofugal fibers showed that about half the ganglion cells (including the displaced ganglion cells) pass through a period of vulnerability to the KA injections, to which they subsequently become sensitive.
Keywords
Animals, Animals, Newborn/growth &amp, development, Animals, Newborn/physiology, Brain/physiology, Chick Embryo/physiology, Chickens/physiology, Kainic Acid/pharmacology, Retina/drug effects, Synaptic Transmission, Time Factors, Visual Pathways/physiology
Pubmed
Web of science
Create date
20/01/2008 18:48
Last modification date
20/08/2019 17:03
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