Automatic segmentation of high pressure frozen and freeze-substituted mouse retina nuclei from FIB-SEM tomograms.

Details

Serval ID
serval:BIB_DF3470F043D1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Automatic segmentation of high pressure frozen and freeze-substituted mouse retina nuclei from FIB-SEM tomograms.
Journal
Journal of structural biology
Author(s)
Hoang T.V., Kizilyaprak C., Spehner D., Humbel B.M., Schultz P.
ISSN
1095-8657 (Electronic)
ISSN-L
1047-8477
Publication state
Published
Issued date
02/2017
Peer-reviewed
Oui
Volume
197
Number
2
Pages
123-134
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Focused Ion Beam milling combined with Scanning Electron Microscopy is a powerful tool to determine the 3-D organization of whole cells and tissue at an isotropic resolution of 3-5nm. This opens the possibility to quantify several cellular parameters and to provide detailed phenotypic information in normal or disease states. Here we describe Biocomputing methods to extract in an automated way characteristic features of mouse rod photoreceptor nuclei such as the shape and the volume of the nucleus; the proportion of heterochromatin; the number, density and distribution of nuclear pore complexes (NPC). Values obtained on five nuclei show that the number of NPC (348±8) is the most conserved feature. Nuclei in higher eukaryotes show large variations in size and rod nuclei are amongst the smallest reported (32±3μm <sup>3</sup> ). Despite large species- and cell-type-specific variations in size, the density of NPC (about 15/μm <sup>2</sup> ) is highly conserved.
Keywords
Animals, Freeze Substitution/methods, Heterochromatin/ultrastructure, Mice, Microscopy, Electron, Scanning/methods, Nuclear Pore Complex Proteins/ultrastructure, Retina/ultrastructure, FIB/SEM tomography, Heterochromatin, Nucleus, Segmentation
Pubmed
Web of science
Create date
19/10/2016 12:52
Last modification date
27/04/2024 7:04
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