Photodynamic therapy with m-tetrahydroxyphenylchlorin in vivo: optimization of the therapeutic index

Details

Serval ID
serval:BIB_DF117AF81CD7
Type
Article: article from journal or magazin.
Collection
Publications
Title
Photodynamic therapy with m-tetrahydroxyphenylchlorin in vivo: optimization of the therapeutic index
Journal
International Journal of Cancer
Author(s)
Ris  H. B., Altermatt  H. J., Stewart  C. M., Schaffner  T., Wang  Q., Lim  C. K., Bonnett  R., Althaus  U.
ISSN
0020-7136 (Print)
Publication state
Published
Issued date
09/1993
Volume
55
Number
2
Pages
245-9
Notes
Journal Article --- Old url value: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8370622 --- Old month value: Sep 9
Abstract
The therapeutic index of meta-tetrahydroxyphenylchlorin-mediated photodynamic therapy (mTHPC-PDT) was assessed in BALB/c nude mice bearing human malignant mesothelioma xenografts. Equal doses of 650 nm laser light were delivered to the tumour and to an equal-sized area of the hind leg (control site) after i.p. administration of mTHPC. Twenty-one groups of 6 animals each were treated under various drug-light conditions and at drug-light intervals ranging from 4 hr to 6 days. After light delivery the extent of tumour necrosis and the depth of alterations in normal tissue were assessed by light microscopy of standardized histological sections. A therapeutic index (TI) of mTHPC-PDT was defined as the cross-sectional area of tumour necrosis per depth of visible tissue injury at the control site. This TI was strongly related to the conditions of treatment. In particular, it was increased by prolonging the drug-light interval up to 5 days and by increasing the dose of light for any dose of drug. The most profound increase of TI was obtained by increasing the intensity of light administered at the chosen interval while reducing the dose of drug. Our findings suggest that threshold conditions operate in PDT and have important implications for clinical application of the treatment.
Keywords
Animals Antineoplastic Agents/*administration & dosage/therapeutic use Dose-Response Relationship, Drug Dose-Response Relationship, Radiation Male Mesoporphyrins/*administration & dosage/therapeutic use Mesothelioma/*drug therapy/pathology Mice Mice, Inbred BALB C Mice, Nude *Photochemotherapy Radiation-Sensitizing Agents/*administration & dosage/therapeutic use Temperature
Pubmed
Web of science
Create date
29/01/2008 13:00
Last modification date
20/08/2019 16:03
Usage data