Targeted development of microsatellite markers from inter-Alu amplification of YAC clones

Details

Serval ID
serval:BIB_DD56E08E3217
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Targeted development of microsatellite markers from inter-Alu amplification of YAC clones
Journal
Genomics
Author(s)
de Souza  A. P., Allamand  V., Richard  I., Brenguier  L., Chumakov  I., Cohen  D., Beckmann  J. S.
ISSN
0888-7543 (Print)
Publication state
Published
Issued date
01/1994
Volume
19
Number
2
Pages
391-3
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan 15
Abstract
Primary genetic maps based on highly informative markers are now available. The local density of these markers may, however, not be sufficient. There is thus a need for new means to generate polymorphic markers from targeted regions of the genome. This can be achieved by selectively cloning and sequencing (CA)n-positive human inter-Alu sequences from targeted YAC clones. This method was tested on 21 YACs and led to the development of seven new polymorphic microsatellite markers.
Keywords
Base Sequence Chromosome Mapping/*methods Chromosomes, Artificial, Yeast Cloning, Molecular DNA, Satellite/*genetics Gene Amplification *Genetic Markers Humans Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Repetitive Sequences, Nucleic Acid
Pubmed
Web of science
Create date
25/01/2008 17:18
Last modification date
20/08/2019 17:02
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