Francisella requires dynamic type VI secretion system and ClpB to deliver effectors for phagosomal escape.

Details

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State: Public
Version: Final published version
Serval ID
serval:BIB_DD0E858F0323
Type
Article: article from journal or magazin.
Collection
Publications
Title
Francisella requires dynamic type VI secretion system and ClpB to deliver effectors for phagosomal escape.
Journal
Nature Communications
Author(s)
Brodmann M., Dreier R.F., Broz P., Basler M.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Publication state
Published
Issued date
2017
Peer-reviewed
Oui
Volume
8
Pages
15853
Language
english
Abstract
Francisella tularensis is an intracellular pathogen that causes the fatal zoonotic disease tularaemia. Critical for its pathogenesis is the ability of the phagocytosed bacteria to escape into the cell cytosol. For this, the bacteria use a non-canonical type VI secretion system (T6SS) encoded on the Francisella pathogenicity island (FPI). Here we show that in F. novicida T6SS assembly initiates at the bacterial poles both in vitro and within infected macrophages. T6SS dynamics and function depends on the general purpose ClpB unfoldase, which specifically colocalizes with contracted sheaths and is required for their disassembly. T6SS assembly depends on iglF, iglG, iglI and iglJ, whereas pdpC, pdpD, pdpE and anmK are dispensable. Importantly, strains lacking pdpC and pdpD are unable to escape from phagosome, activate AIM2 inflammasome or cause disease in mice. This suggests that PdpC and PdpD are T6SS effectors involved in phagosome rupture.

Pubmed
Web of science
Open Access
Yes
Create date
28/11/2017 14:48
Last modification date
20/08/2019 17:01
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