Actin cables and the exocyst form two independent morphogenesis pathways in the fission yeast.

Details

Ressource 1Download: BIB_DCBEA5F67977.P001.pdf (8129.71 [Ko])
State: Public
Version: Final published version
Serval ID
serval:BIB_DCBEA5F67977
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Actin cables and the exocyst form two independent morphogenesis pathways in the fission yeast.
Journal
Molecular Biology of the Cell
Author(s)
Bendezú F.O., Martin S.G.
ISSN
1939-4586 (Electronic)
ISSN-L
1059-1524
Publication state
Published
Issued date
2011
Peer-reviewed
Oui
Volume
22
Number
1
Pages
44-53
Language
english
Abstract
Cell morphogenesis depends on polarized exocytosis. One widely held model posits that long-range transport and exocyst-dependent tethering of exocytic vesicles at the plasma membrane sequentially drive this process. Here, we describe that disruption of either actin-based long-range transport and microtubules or the exocyst did not abolish polarized growth in rod-shaped fission yeast cells. However, disruption of both actin cables and exocyst led to isotropic growth. Exocytic vesicles localized to cell tips in single mutants but were dispersed in double mutants. In contrast, a marker for active Cdc42, a major polarity landmark, localized to discreet cortical sites even in double mutants. Localization and photobleaching studies show that the exocyst subunits Sec6 and Sec8 localize to cell tips largely independently of the actin cytoskeleton, but in a cdc42 and phospholipid phosphatidylinositol 4,5-bisphosphate (PIP₂)-dependent manner. Thus in fission yeast long-range cytoskeletal transport and PIP₂-dependent exocyst represent parallel morphogenetic modules downstream of Cdc42, raising the possibility of similar mechanisms in other cell types.
Keywords
Actins/metabolism, Cell Polarity, Cell Proliferation, Cytoskeleton/physiology, Exocytosis, Fluorescent Dyes, Microfilaments/genetics, Microfilaments/metabolism, Microtubules/genetics, Microtubules/metabolism, Morphogenesis, Phosphoinositide Phospholipase C/metabolism, Schizosaccharomyces/cytology, Schizosaccharomyces/growth & development, Schizosaccharomyces pombe Proteins/genetics, Schizosaccharomyces pombe Proteins/metabolism, Vesicular Transport Proteins/metabolism, cdc42 GTP-Binding Protein/metabolism
Pubmed
Web of science
Create date
12/01/2011 13:13
Last modification date
20/08/2019 16:01
Usage data