Questioning the role of selected somatic PIK3C2B mutations in squamous non-small cell lung cancer oncogenesis.

Details

Serval ID
serval:BIB_DC869998EDA7
Type
Article: article from journal or magazin.
Collection
Publications
Title
Questioning the role of selected somatic PIK3C2B mutations in squamous non-small cell lung cancer oncogenesis.
Journal
PloS one
Author(s)
Kind M., Klukowska-Rötzler J., Berezowska S., Arcaro A., Charles R.P.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2017
Peer-reviewed
Oui
Volume
12
Number
10
Pages
e0187308
Language
english
Notes
Publication types: Journal Article ; Validation Study
Publication Status: epublish
Abstract
PI3K signaling is frequently dysregulated in NSCLC-SQCC. In contrast to well characterized components of the PI3K signaling network contributing to the formation of SQCC, potential oncogenic effects of alterations in PIK3C2B are poorly understood. Here, a large cohort (n = 362) of NSCLC-SQCC was selectively screened for four reported somatic mutations in PIK3C2B via Sanger sequencing. In addition, two mutations leading to an amino acid exchange in the kinase domain (C1181, H1208R) were examined on a functional level. None of the mutations were identified in the cohort while well characterized hotspot PIK3CA mutations were observed at the expected frequency. Ultimately, kinase domain mutations in PI3KC2β were found to have no altering effect on downstream signaling. A set of SQCC tumors sequenced by The Cancer Genome Atlas (TCGA) equally indicates a lack of oncogenic potential of the kinase domain mutations or PIK3C2B in general. Taken together, this study suggests that PIK3C2B might only have a minor role in SQCC oncogenesis.
Keywords
Carcinogenesis, Carcinoma, Non-Small-Cell Lung/genetics, Carcinoma, Non-Small-Cell Lung/pathology, Class II Phosphatidylinositol 3-Kinases/genetics, Cohort Studies, HEK293 Cells, Humans, Lung Neoplasms/genetics, Lung Neoplasms/pathology, Mutation
Pubmed
Web of science
Open Access
Yes
Create date
29/06/2020 10:29
Last modification date
30/06/2020 5:26
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