Structure and evolutionary origin of the human granzyme H gene

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Serval ID
serval:BIB_DC4FFD80165F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Structure and evolutionary origin of the human granzyme H gene
Journal
International Immunology
Author(s)
Haddad  P., Jenne  D., Tschopp  J., Clement  M. V., Mathieu-Mahul  D., Sasportes  M.
ISSN
0953-8178 (Print)
Publication state
Published
Issued date
01/1991
Volume
3
Number
1
Pages
57-66
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan
Abstract
Among the molecules proposed to be involved in cytotoxic T lymphocyte (CTL), natural killer (NK) and lymphokine activated killer (LAK) cell-mediated lysis are the granzymes, a family of serine proteases stored in the cytoplasmic granules of CTLs, NK and LAK cells. In addition to the granzymes A and B, a third member of this family has been cloned in man and designated granzyme H. We present the complete gene sequence including the 5' promoter region and demonstrate that the granzyme H sequence represents a functional gene expressed in activated T cells. Granzyme H shows the highest degree (greater than 54%) of amino acid sequence homology with granzyme B and cathepsin G and, like these genes, consists of five exons separated by introns at equivalent positions. The evolutionary history of granzyme H has been analyzed by reconstructing an evolutionary tree for granzyme sequences. We provide evidence that interlocus recombination between the ancestral genes of granzyme B and granzyme H occurred about 21 million years ago, leading to a replacement of exon 3, intron 3 and part of exon 4 in human granzyme H by human granzyme B sequences. Our results suggest that the ancestral gene of granzyme H is more closely related to cathepsin G and granzyme B than to the murine granzymes C to G. Thus, granzyme H does not represent a human counterpart of the known murine granzymes A to G. It diverged from cathepsin G before mammalian radiation and should, therefore, exist in other mammalian lineages as well.
Keywords
Amino Acid Sequence Base Sequence Cathepsins/genetics DNA/genetics Evolution Gene Expression Granzymes Humans Lymphocytes/enzymology Molecular Sequence Data Restriction Mapping Sequence Homology, Nucleic Acid Serine Endopeptidases/*genetics
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 16:19
Last modification date
01/10/2019 7:19
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