A Miniaturized Microbe-Silicon-Chip Based on Bioluminescent Engineered Escherichia coli for the Evaluation of Water Quality and Safety.

Details

Serval ID
serval:BIB_DC12ED645274
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A Miniaturized Microbe-Silicon-Chip Based on Bioluminescent Engineered Escherichia coli for the Evaluation of Water Quality and Safety.
Journal
International journal of environmental research and public health
Author(s)
Sciuto E.L., Corso D., Libertino S., van der Meer J.R., Faro G., Coniglio M.A.
ISSN
1660-4601 (Electronic)
ISSN-L
1660-4601
Publication state
Published
Issued date
16/07/2021
Peer-reviewed
Oui
Volume
18
Number
14
Pages
7580
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Conventional high throughput methods assaying the chemical state of water and the risk of heavy metal accumulation share common constraints of long and expensive analytical procedures and dedicated laboratories due to the typical bulky instrumentation. To overcome these limitations, a miniaturized optical system for the detection and quantification of inorganic mercury (Hg <sup>2+</sup> ) in water was developed. Combining the bioactivity of a light-emitting mercury-specific engineered Escherichia coli-used as sensing element-with the optical performance of small size and inexpensive Silicon Photomultiplier (SiPM)-used as detector-the system is able to detect mercury in low volumes of water down to the concentration of 1 µg L <sup>-1</sup> , which is the tolerance value indicated by the World Health Organization (WHO), providing a highly sensitive and miniaturized tool for in situ water quality analysis.
Keywords
Escherichia coli/genetics, Mercury/analysis, Water, Water Pollutants, Chemical/analysis, Water Quality, SiPM, engineered Escherichia coli, mercury sensing, miniaturizable optical system, water analysis
Pubmed
Web of science
Open Access
Yes
Create date
02/08/2021 15:04
Last modification date
23/02/2022 7:36
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