Combination of Hyaluronan and Lyophilized Progenitor Cell Derivatives: Stabilization of Functional Hydrogel Products for Therapeutic Management of Tendinous Tissue Disorders.

Details

Serval ID
serval:BIB_DBCEF906F288
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Combination of Hyaluronan and Lyophilized Progenitor Cell Derivatives: Stabilization of Functional Hydrogel Products for Therapeutic Management of Tendinous Tissue Disorders.
Journal
Pharmaceutics
Author(s)
Laurent A., Porcello A., Fernandez P.G., Jeannerat A., Peneveyre C., Abdel-Sayed P., Scaletta C., Hirt-Burri N., Michetti M., de Buys Roessingh A., Raffoul W., Allémann E., Jordan O., Applegate L.A.
ISSN
1999-4923 (Print)
ISSN-L
1999-4923
Publication state
Published
Issued date
19/12/2021
Peer-reviewed
Oui
Volume
13
Number
12
Pages
2196
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Cultured progenitor cells and derivatives have been used in various homologous applications of cutaneous and musculoskeletal regenerative medicine. Active pharmaceutical ingredients (API) in the form of progenitor cell derivatives such as lysates and lyophilizates were shown to retain function in controlled cellular models of wound repair. On the other hand, hyaluronan-based hydrogels are widely used as functional vehicles in therapeutic products for tendon tissue disorders. The aim of this study was the experimental characterization of formulations containing progenitor tenocyte-derived APIs and hyaluronan, for the assessment of ingredient compatibility and stability in view of eventual therapeutic applications in tendinopathies. Lyophilized APIs were determined to contain relatively low quantities of proteins and growth factors, while being physicochemically stable and possessing significant intrinsic antioxidant properties. Physical and rheological quantifications of the combination formulas were performed after hydrogen peroxide challenge, outlining significantly improved evolutive viscoelasticity values in accelerated degradation settings. Thus, potent effects of physicochemical protection or stability enhancement of hyaluronan by the incorporated APIs were observed. Finally, combination formulas were found to be easily injectable into ex vivo tendon tissues, confirming their compatibility with further translational clinical approaches. Overall, this study provides the technical bases for the development of progenitor tenocyte derivative-based injectable therapeutic products or devices, to potentially be applied in tendinous tissue disorders.
Keywords
cell therapies, hyaluronic acid, hydrogels, progenitor cells, regenerative medicine, stabilization, tendinopathies, viscoelasticity
Pubmed
Web of science
Open Access
Yes
Create date
03/01/2022 16:54
Last modification date
15/01/2022 7:32
Usage data