Differential effects of pro- and anti-inflammatory cytokines alone or in combinations on the metabolic profile of astrocytes.

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State: Public
Version: author
Serval ID
serval:BIB_DBA12B8DF103
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Differential effects of pro- and anti-inflammatory cytokines alone or in combinations on the metabolic profile of astrocytes.
Journal
Journal of Neurochemistry
Author(s)
Bélanger M., Allaman I., Magistretti P.J.
ISSN
1471-4159[electronic], 0022-3042[linking]
Publication state
Published
Issued date
2011
Peer-reviewed
Oui
Volume
116
Number
4
Pages
564-576
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication
Abstract
We have previously reported that the pro-inflammatory cytokines tumor necrosis factor-α (TNFα) and interleukin-1β (IL-1β) induce profound modifications of the metabolic profile of astrocytes. The present study was undertaken to further characterize the effects of cytokines in astrocytes and to determine whether similar effects could also be observed in neurons. To do so, selected pro-inflammatory (IL-6 and interferon-γ, in addition to the above-mentioned TNFα and IL-1β) and anti-inflammatory cytokines (IL-4, IL-10, transforming growth factor-β1 and interferon-β) were applied to primary neuronal and astrocytic cultures, and key metabolic parameters were assessed. As a general pattern, we observed that pro-inflammatory cytokines increased glucose utilization in astrocytes while the anti-inflammatory cytokines IL-4 and IL-10 decreased astrocytic glucose utilization. In contrast, no significant change could be observed in neurons. When pairs of pro-inflammatory cytokines were co-applied in astrocytes, several additive or synergistic modifications could be observed. In contrast, IL-10 partially attenuated the effects of pro-inflammatory cytokines. Finally, the modifications of the astrocytic metabolism induced by TNFα + IL-1β and interferon-γ modulated neuronal susceptibility to an excitotoxic insult in neuron-astrocyte co-cultures. Together, these results suggest that pro- and anti-inflammatory cytokines differentially affect the metabolic profile of astrocytes, and that these changes have functional consequences for surrounding neurons.
Pubmed
Web of science
Open Access
Yes
Create date
11/03/2011 16:00
Last modification date
20/08/2019 17:00
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