Postnatal expression pattern of calcium-binding proteins in organotypic thalamic cultures and in the dorsal thalamus in vivo.

Details

Serval ID
serval:BIB_DB533E06A9D2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Postnatal expression pattern of calcium-binding proteins in organotypic thalamic cultures and in the dorsal thalamus in vivo.
Journal
Brain Research. Developmental Brain Research
Author(s)
Sieg F., Obst K., Gorba T., Riederer B., Pape H.C., Wahle P.
ISSN
0165-3806 (Print)
ISSN-L
0165-3806
Publication state
Published
Issued date
1998
Volume
110
Number
1
Pages
83-95
Language
english
Abstract
The present study describes the postnatal expression of calbindin, calretinin and parvalbumin and glutamic acid decarboxylase (GAD) and microtubule-associated protein 2 (MAP2) in organotypic monocultures of rat dorsal thalamus compared to the thalamus in vivo. Cultures were maintained for up to 7 weeks. Cortex-conditioned medium improved the survival of thalamic cultures. MAP2-immunoreactive material was present in somata and dendrites of small and large-sized neurons throughout the cultures. Parvalbumin immunoreactivity was present in larger multipolar or bitufted neurons along the edge of a culture. These neurons also displayed strong parvalbumin mRNA and GAD mRNA expression, and GABA immunoreactivity. They likely corresponded to cells of the nucleus reticularis thalami. Parvalbumin mRNA, but neither parvalbumin protein nor GAD mRNA, was expressed in neurons with large somata within the explant. They likely represented relay cells. GAD mRNA, but not parvalbumin mRNA, was expressed in small neurons within the explants. Small neurons also displayed calbindin- and calretinin-immunoreactivity. The small neurons likely represented local circuit neurons. The time course of expression of the calcium-binding proteins revealed that all were present at birth with the predicted molecular weights. A low, but constant parvalbumin expression was observed in vitro without the developmental increase seen in vivo, which most likely represented parvalbumin from afferent sources. In contrast, the explantation transiently downregulated the calretinin and calbindin expression, but the neurons recovered the expression after 14 and 21 days, respectively. In conclusion, thalamic monocultures older than three weeks represent a stable neuronal network containing well differentiated neurons of the nucleus reticularis thalami, relay cells and local circuit neurons.
Keywords
Aging/metabolism, Animals, Animals, Newborn, Calcium-Binding Protein, Vitamin D-Dependent/genetics, Calcium-Binding Proteins/biosynthesis, Calcium-Binding Proteins/genetics, Cerebral Cortex/physiology, Culture Media, Conditioned, Gene Expression Regulation, Developmental, Glutamate Decarboxylase/genetics, Microtubule-Associated Proteins/genetics, Nerve Tissue Proteins/genetics, Organ Culture Techniques, Parvalbumins/genetics, RNA, Messenger/biosynthesis, RNA, Messenger/genetics, Rats, Rats, Long-Evans, Thalamus/growth & development, Thalamus/metabolism, Transcription, Genetic
Pubmed
Create date
24/01/2008 14:34
Last modification date
20/08/2019 16:00
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