Tightly regulated expression of the entire hepatitis C virus structural region in continuous human cell lines
Details
Serval ID
serval:BIB_DB4A1A8236E2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Tightly regulated expression of the entire hepatitis C virus structural region in continuous human cell lines
Journal
Biochemical and Biophysical Research Communications
ISSN
0006-291X (Print)
Publication state
Published
Issued date
05/1998
Volume
246
Number
3
Pages
920-4
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May 29
Research Support, Non-U.S. Gov't --- Old month value: May 29
Abstract
Investigation of the hepatitis C virus (HCV) life cycle is limited by the lack of an efficient cell culture system. Employing a tetracycline-regulated gene expression system we generated a panel of continuous human cell lines allowing the inducible expression and faithful processing of HCV structural proteins as well as of a functional NS2-3 autoprotease. HCV proteins were found in the cytoplasm in a pattern characteristic for the endoplasmic reticulum. High-level expression of HCV proteins was found to be cytotoxic. These cell lines represent a unique in vitro system in which to further investigate the structural proteins of HCV and to evaluate novel antiviral strategies against hepatitis C in a well-defined and reproducible cellular context.
Keywords
Cell Compartmentation
Cell Division
Cell Survival
Chromosomes, Human
*Gene Expression Regulation, Viral
Hepacivirus/*growth & development
Humans
Transgenes
Tumor Cells, Cultured
Viral Structural Proteins/biosynthesis
Virus Cultivation/*methods
Virus Integration
Pubmed
Web of science
Create date
25/01/2008 17:05
Last modification date
20/08/2019 17:00