Effect of amino acids and glucose on exercise-induced gut and skeletal muscle proteolysis in dogs.

Details

Serval ID
serval:BIB_DB033452F866
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Effect of amino acids and glucose on exercise-induced gut and skeletal muscle proteolysis in dogs.
Journal
Metabolism: Clinical and Experimental
Author(s)
Hamada K., Matsumoto K., Okamura K., Doi T., Minehira K., Shimizu S.
ISSN
0026-0495
Publication state
Published
Issued date
1999
Peer-reviewed
Oui
Volume
48
Number
2
Pages
161-6
Language
english
Abstract
The effect of amino acid and/or glucose administration before and during exercise on protein metabolism in visceral tissues and skeletal muscle was examined in mongrel dogs. The dogs were subjected to treadmill running (150 minutes at 10 km/h and 12% incline) and intravenously infused with a solution containing amino acids and glucose (AAG), amino acids (AA), glucose (G) or saline (S) in randomized order. The infusion was started 60 minutes before exercise and continued until the end of the exercise period. An arteriovenous-difference technique was used to estimate both tissue protein degradation and synthesis. When S was infused, the release of leucine (Leu) from the gut and phenylalanine (Phe) from the hindlimb significantly increased during exercise, thus indicating that exercise augmented proteolysis in these tissues. The balance of Leu across the gut during exercise demonstrated a net uptake with both AAG and AA, whereas a net release was observed for G and S. In addition, Leu uptake in the gut during the last 90 minutes of the exercise period tended to be greater with AAG versus AA (P = .06). Phe balance across the hindlimb during the late exercise period showed a significant release with S, AA, and G, whereas the balance with AAG did not show a significant release. These results suggest that exercise-induced proteolysis in the gut may be reduced by supplementation with AA, and this effect may be enhanced by concomitant G administration. However, in skeletal muscle, both AA and G may be required to prevent net protein degradation during exercise. G provided without AA did not achieve net protein synthesis in either tissue.
Keywords
Amino Acids, Animals, Blood Glucose, Dogs, Glucose, Inulin, Leucine, Male, Muscle Proteins, Muscle, Skeletal, Muscle, Smooth, Phenylalanine, Physical Exertion, Regional Blood Flow
Pubmed
Web of science
Create date
20/02/2009 12:43
Last modification date
20/08/2019 16:00
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