Estrella lausannensis, a new star in the Chlamydiales order.
Details
State: Public
Version: author
Serval ID
serval:BIB_DAC8F9D68874
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Estrella lausannensis, a new star in the Chlamydiales order.
Journal
Microbes and Infection / Institut Pasteur
ISSN
1769-714X (Electronic)
ISSN-L
1286-4579
Publication state
Published
Issued date
2011
Peer-reviewed
Oui
Volume
13
Number
14-15
Pages
1232-1241
Language
english
Abstract
Originally, the Chlamydiales order was represented by a single family, the Chlamydiaceae, composed of several pathogens, such as Chlamydia trachomatis, Chlamydia pneumoniae, Chlamydia psittaci and Chlamydia abortus. Recently, 6 new families of Chlamydia-related bacteria have been added to the Chlamydiales order. Most of these obligate intracellular bacteria are able to replicate in free-living amoebae. Amoebal co-culture may be used to selectively isolate amoeba-resisting bacteria. This method allowed in a previous work to discover strain CRIB 30, from an environmental water sample. Based on its 16S rRNA gene sequence similarity with Criblamydia sequanensis, strain CRIB 30 was considered as a new member of the Criblamydiaceae family. In the present work, phylogenetic analyses of the genes gyrA, gyrB, rpoA, rpoB, secY, topA and 23S rRNA as well as MALDI-TOF MS confirmed the taxonomic classification of strain CRIB 30. Morphological examination revealed peculiar star-shaped elementary bodies (EBs) similar to those of C. sequanensis. Therefore, this new strain was called "Estrella lausannensis". Finally, E. lausannensis showed a large amoebal host range and a very efficient replication rate in Acanthamoeba species. Furthermore, E. lausannensis is the first member of the Chlamydiales order to grow successfully in the genetically tractable Dictyostelium discoideum, which opens new perspectives in the study of chlamydial biology.
Pubmed
Web of science
Open Access
Yes
Create date
17/02/2012 9:22
Last modification date
20/08/2019 16:59