Characterization of the sex determining region of channel catfish (Ictalurus punctatus) and development of a sex-genotyping test.

Details

Serval ID
serval:BIB_DAAE2809C02D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Characterization of the sex determining region of channel catfish (Ictalurus punctatus) and development of a sex-genotyping test.
Journal
Gene
Author(s)
Wen M., Pan Q., Larson W., Eché C., Guiguen Y.
ISSN
1879-0038 (Electronic)
ISSN-L
0378-1119
Publication state
Published
Issued date
30/09/2022
Peer-reviewed
Oui
Volume
850
Pages
146933
Language
english
Notes
Publication types: Journal Article
Publication Status: aheadofprint
Abstract
Channel catfish is an important species for aquaculture that exhibits a sexually dimorphic growth in favor of males. Genetic sexing and development of sex markers are crucial for the early identification of sex and of particular genotypes (YY males) for the production of all-male population in channel catfish aquaculture. In this study, we sequenced genomic DNA from pools of males and pools of females to better characterize the sex determining region (SDR) of channel catfish and to develop sex-specific markers for genetic sexing. Performing comparative analyses on male and female pooled genomic reads, we identified a large SDR (∼8.3 Mb) in the middle of channel catfish linkage group 4 (LG04). This non-recombining SDR contains a high-density of male-specific (Y chromosome) fixed single nucleotide polymorphisms (SNPs) along with ∼ 185 kb male-specific insertions or deletions. This SDR contains 95 annotated protein-encoding genes, including the recently reported putative channel catfish master sex determining (MSD) gene, breast cancer anti-estrogen resistance protein 1 (bcar1), located at one edge of the SDR. No sex-specific SNPs and/or indels were found in the coding sequence of bcar1, but one male-specific SNP was identified in its first intron. Based on this genomic information, we developed a PCR-based sex-specific genetic test. Genotyping results confirmed strong linkage between phenotypic sexes and the identified SDR in channel catfish. Our results confirm, using a Pool-Seq approach, that channel catfish is male heterogametic (XX-XY) with a large SDR on the LG04 sex chromosome. Furthermore, our genotyping primers can be used to identify XX, XY, and YY fish that will facilitate future research on sex determination and aquaculture applications in channel catfish.
Keywords
Channel catfish, Pool-sequencing, Sex chromosome, Sex determining region, Sex markers
Pubmed
Create date
10/10/2022 13:33
Last modification date
12/10/2022 5:39
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