Binding and transepithelial transport of immunoglobulins by intestinal M cells: demonstration using monoclonal IgA antibodies against enteric viral proteins

Details

Serval ID
serval:BIB_DA81C103392E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Binding and transepithelial transport of immunoglobulins by intestinal M cells: demonstration using monoclonal IgA antibodies against enteric viral proteins
Journal
Journal of Cell Biology
Author(s)
Weltzin  R., Lucia-Jandris  P., Michetti  P., Fields  B. N., Kraehenbuhl  J. P., Neutra  M. R.
ISSN
0021-9525 (Print)
Publication state
Published
Issued date
05/1989
Volume
108
Number
5
Pages
1673-85
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: May
Abstract
M cells of intestinal epithelia overlying lymphoid follicles endocytose luminal macromolecules and microorganisms and deliver them to underlying lymphoid tissue. The effect of luminal secretory IgA antibodies on adherence and transepithelial transport of antigens and microorganisms by M cells is unknown. We have studied the interaction of monoclonal IgA antibodies directed against specific enteric viruses, or the hapten trinitrophenyl (TNP), with M cells. To produce monospecific IgA antibodies against mouse mammary tumor virus (MMTV) and reovirus type 1, Peyer's patch cells from mucosally immunized mice were fused with myeloma cells, generating hybridomas that secreted virus-specific IgA antibodies in monomeric and polymeric forms. One of two anti-MMTV IgA antibodies specifically bound the viral surface glycoprotein gp52, and 3 of 10 antireovirus IgA antibodies immunoprecipitated sigma 3 and mu lc surface proteins. 35S-labeled IgA antibodies injected intravenously into rats were recovered in bile as higher molecular weight species, suggesting that secretory component had been added on passage through the liver. Radiolabeled or colloidal gold-conjugated mouse IgA was injected into mouse, rat, and rabbit intestinal loops containing Peyer's patches. Light microscopic autoradiography and EM showed that all IgA antibodies (antivirus or anti-TNP) bound to M cell luminal membranes and were transported in vesicles across M cells. IgA-gold binding was inhibited by excess unlabeled IgA, indicating that binding was specific. IgG-gold also adhered to M cells and excess unlabeled IgG inhibited IgA-gold binding; thus binding was not isotype-specific. Immune complexes consisting of monoclonal anti-TNP IgA and TNP-ferritin adhered selectively to M cell membranes, while TNP-ferritin alone did not. These results suggest that selective adherence of luminal antibody to M cells may facilitate delivery of virus-antibody complexes to mucosal lymphoid tissue, enhancing subsequent secretory immune responses or facilitating viral invasion.
Keywords
Animals Antibodies, Monoclonal/*immunology Antigen-Antibody Reactions Autoradiography Epithelium/immunology Female Fluorescent Antibody Technique Immunoglobulin A/*immunology Immunoglobulins/*metabolism Intestinal Mucosa/cytology/*immunology/ultrastructure Liver/*immunology Male Mammary Tumor Virus, Mouse/*immunology Mice Mice, Inbred BALB C Microscopy, Electron Peyer's Patches/cytology/*immunology/ultrastructure Rats Receptors, Immunologic/*metabolism Reoviridae/*immunology Sulfur Radioisotopes Viral Proteins/*immunology
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 16:02
Last modification date
20/08/2019 15:59
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